Transmembrane potential induced on the internal organelle by a time-varying magnetic field: a model study
© Ye et al; licensee BioMed Central Ltd. 2010
Received: 16 September 2009
Accepted: 20 February 2010
Published: 20 February 2010
When a cell is exposed to a time-varying magnetic field, this leads to an induced voltage on the cytoplasmic membrane, as well as on the membranes of the internal organelles, such as mitochondria. These potential changes in the organelles could have a significant impact on their functionality. However, a quantitative analysis on the magnetically-induced membrane potential on the internal organelles has not been performed.
Using a two-shell model, we provided the first analytical solution for the transmembrane potential in the organelle membrane induced by a time-varying magnetic field. We then analyzed factors that impact on the polarization of the organelle, including the frequency of the magnetic field, the presence of the outer cytoplasmic membrane, and electrical and geometrical parameters of the cytoplasmic membrane and the organelle membrane.
The amount of polarization in the organelle was less than its counterpart in the cytoplasmic membrane. This was largely due to the presence of the cell membrane, which "shielded" the internal organelle from excessive polarization by the field. Organelle polarization was largely dependent on the frequency of the magnetic field, and its polarization was not significant under the low frequency band used for transcranial magnetic stimulation (TMS). Both the properties of the cytoplasmic and the organelle membranes affect the polarization of the internal organelle in a frequency-dependent manner.
The work provided a theoretical framework and insights into factors affecting mitochondrial function under time-varying magnetic stimulation, and provided evidence that TMS does not affect normal mitochondrial functionality by altering its membrane potential.
Time-varying magnetic fields have been used to stimulate neural tissues since the start of 20th century . Today, pulsed magnetic fields are used in stimulating the central nervous system, via a technique named transcranial magnetic stimulation (TMS). TMS is being explored in the treatment of depression , seizures [3, 4], Parkinson's disease , and Alzheimer's disease [6, 7]. It also facilitates long-lasting plastic changes induced by motor practice, leading to more remarkable and outlasting clinical gains during recovery from stroke or traumatic brain injury .
When exposed to a time-varying magnetic field, the neural tissue is stimulated by an electric current via electromagnetic induction , which induces an electrical potential that is superimposed on the resting membrane potential of the cell. The polarization could be controlled by appropriate geometrical positioning of the magnetic coil [10–12]. To investigate the effects of stimulation, theoretical studies have been performed to compute the magnetically induced electric field and potentials in the neuronal tissue, using models that represent nerve fibers [13–18] or cell bodies .
Mitochondria are involved in a large range of physiological processes such as supplying cellular energy, signaling, cellular differentiation, cell death, as well as the control of cell cycle and growth . Their large negative membrane potential (-180 mV) in the mitochondrial inner membrane, which is generated by the electron-transport chain, is the main driving force in these regulatory processes [21–23]. Alteration of this large negative membrane potential has been associated with disruption in cellular homeostasis that leads to cell death in aging and many neurological disorders [24–27]. Thus, mitochondria can be a therapeutic target in many neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease.
Two lines of evidences suggest that the physiology of mitochondria could be affected by the magnetic field via its induced transmembrane potential. First, magnetic fields can induce electric fields in the neural tissue, and it has been shown that exposure of a cell to an electrical field could introduce a voltage on the mitochondrial membrane . This induced potential has led to many physiological/pathological changes, such as opening of the mitochondrial permeability transition pore complex . Nanosecond pulsed electric fields (nsPEFs) can affect mitochondrial membrane [30, 31], cause calcium release from internal stores , and induce mitochondria-dependent apoptosis under severe stress [33, 34]. Secondly, there is evidence that magnetic fields could alter several important physiological processes that are related to the mitochondrial membrane potential, including ATP synthesis [35, 36], metabolic activities [37, 38] and Ca2+ handling [39, 40]. An analysis of the mitochondrial membrane potential is of experimental significance in understanding its physiology/pathology under magnetic stimulation.
In this theoretical work, we have provided the first analytical solution for the transmembrane potential in an internal organelle (i.e., mitochondrion) that is induced by a time-varying magnetic field. The model was a two-shell cell structure, with the outer shell representing the cell membrane and the inner shell representing the membrane of an internal organelle. Factors that affect the amount of organelle polarization were investigated by parametric analysis, including field frequency, and properties of the cytoplasmic and organelle membranes. We also estimated to what degree magnetic fields used in TMS practice affect organelle polarization.
Spherical cell and internal organelle model in a time-varying magnetic field
The co-centric spherical cell and the organelle were represented in a spherical coordinate system (r, θ, ϕ) centered at point O. The cell membrane was represented as a very thin shell with inner radius R-, outer radius R+ and thickness D. The organelle membrane was represented as a very thin shell with inner radius r-, outer radius r+ and thickness d. The two membrane shells divided the cellular environment into five homogenous, isotropic regions: extracellular medium (0#), cytoplasm membrane (1#), intracellular cytoplasm (2#), organelle membrane (3#) and the organelle internal (4#). The dielectric permittivities and the conductivities in the five regions were ε i and σ i , respectively, where i represents the region number.
The low-frequency magnetic field was represented in a cylindrical coordinate system (r', ϕ', z'). The distance between the center of the cell (O) and the axis of the coil (O') was C. The externally applied, sinusoidally alternating magnetic field was symmetric about the O' Z' axis. The magnetic field was represented as , where was the unit vector in the direction of O' Z', ω was the angular frequency of the magnetic field, and was the imaginary unit.
Extracellular conductivity (σ0, S/m)
Cell membrane conductivity (σ1, S/m)
3 × 10-7
1.0 × 10-8
1.0 × 10-6
Cytoplasmic conductivity (σ2, S/m)
Mitochondrion membrane conductivity (σ3, S/m)
3 × 10-7
1.0 × 10-8
1.0 × 10-5
Mitochondrion internal conductivity (σ4, S/m)
Extracellular dielectric permittivity (ε0, As/Vm)
6.4 × 10-10
Cell membrane dielectric permittivity (ε1, As/Vm)
4.4 × 10-11
1.8 × 10-11
8.8 × 10-11
Cytoplasmic dielectric permittivity (ε2, As/Vm)
6.4 × 10-10
3.5 × 10-10
7.0 × 10-10
Mitochondrion membrane permittivity (ε3, As/Vm)
4.4 × 10-11
1.8 × 10-11
8.8 × 10-11
Mitochondrion internal permittivity (ε4, As/Vm)
6.4 × 10-10
3.5 × 10-10
7.0 × 10-10
Cell radius (R, um)
Cell membrane thickness (D, nm)
Mitochondrion radius (r, um)
Mitochondrion membrane thickness (d, nm)
Magnetic field intensity (B0, Tesla)
Magnetic field frequency (f, kHz)
Governing equations for potentials and electric fields induced by the time-varying magnetic field
Four boundary conditions were considered in the derivation of the potentials induced by the time-varying magnetic field.
(A). The potential was continuous across the boundary of two different media. In this paper, this refers to the extracellular media/membrane interface (0#1#), the cell membrane/intracellular cytoplasm interface (1#2#), the intracellular cytoplasm/organelle membrane interface (2#3#), and the organelle membrane/organelle interior interface (3#4#).
where S0 = σ0+jωε0, S1 = σ1+jωε1, S2 = σ2+jωε2, S3 = σ3+jωε3 and S4 = σ4+jωε4 were the complex conductivities of the five media, respectively.
(C). The electric field at an infinite distance from the cell was not perturbed by the presence of the cell.
(D). The potential inside the organelle (r = 0) was finite.
Magnetic vector potential
Derivations of the equations were done with Mathematica 6.0 (Wolfram Research, Inc. Champaign, IL). Numerical simulations were done with Matlab 7.4.0 (The MathWorks, Inc. Natick, MA).
Transmembrane potentials induced by a time-varying magnetic field
where C n , D n were unknown coefficients (n = 0,1,2,3,4,5). We solved for those coefficients (Appendix) and substituted them into equation (13) to obtain the potential terms in the five model regions. Next, the transmembrane potential in a membrane can be obtained by subtracting the membrane potential at the inner surface from that at the outer surface.
Similar regional polarization patterns were observed between the cell membrane and the organelle membrane, since they both depended on a sinθ cosϕ term. Since θ and ϕ were determined by the relative orientation of the coil to the cell, the patterns of polarization in the target cell and the organelle both depended on their orientations to the stimulation coil.
Additional file 1: Dynamic membrane potential changes in the cell and in the internal organelle. A movie that shows the membrane potentials in the cell and in the organelle, induced by a 100 KHz magnetic field. (AVI 207 KB)
Both ψ cell and ψ org depended on the geometrical parameters of the cell (R+, R-, C) and the organelle (r+, r-), and the electrical properties of the five media considered in the model (S0, S1, S2, S3, S4). These parameters did not affect the polarization pattern. Therefore, we chose maximal polarizations (corresponding to the point that is defined by θ = 90°, ϕ = 270°) on the cell and organelle membranes (Figures 1 and 2) for the further analysis of their dependency on the field frequency.
Two factors contribute to the frequency-dependency of the polarizations (magnitude and phase) in the two membranes. First, the magnitude of the electrical field is proportional to the frequency of the externally applied magnetic field, as required by Faraday's law. Second, the dielectric properties of the material considered in the model are frequency-dependent.
The phase was defined as the phase difference between the externally applied magnetic field and membrane polarization, which was computed as the phase angle of the complex transmembrane potentials. Phase in the cell membrane was insensitive to the frequency change below 10 KHz. At 10 KHz, the phase in the cell membrane is -91.23°, which meant that an extra -1.23° was added to the membrane phase, due to frequency-dependent capacitive features of the tissue. On the other hand, phase response in the organelle membrane was more sensitive to the frequency change than the cell membrane, showing the dependence as low as 50 Hz. At 10 KHz, the phase in the organelle was -5.69°. Above 10 KHz, phases in both membranes increased with frequency. At 200 KHz, the phase in the cell membrane was -113.1°, and in the organelle membrane was -33.07°. Figure 3D plots the difference between the two phases as a function of frequency. At very low frequency (< 50 Hz), the two membranes demonstrated an in-phase polarization. At 10 KHz, their polarizations were nearly 90° out-of-phase.
"Interaction" between the cell membrane and the organelle membrane
Previous studies have shown that the cell membrane "shields" the internal cytoplasm and prevent the external field from penetrating inside the cell in electric stimulation [48, 49]. Will similar phenomenon occur under magnetic stimulation? To estimate the impact of cell membrane on organelle polarization, we compared ψ org with and without the presence of the cell membrane. The later was done by letting S1 = S0 and S2 = S0 in equation (15), which removed the cell membrane,
The phase response for the isolated organelle was similar to a cell membrane that was directly exposed in the field (Figure 4B). Therefore, presence of the cell membrane not only" shielded" the internal mitochondria from excessive polarization by the external field, but also provides an extra phase term that reduce the phase delay between the field and the organelle response.
Dependency of ψ org on the cell membrane parameters
At a low frequency band (< 10 KHz), ψ org was trivial, since the magnitude of the induced electric field was small. ψ org became considerably large beyond 10 KHz. Increase in the cell radius facilitates this polarization (Figure 6A left). Increase in the cell radius did not significantly change the phase-frequency relation in the organelle. However, it increased the phase at relatively high frequency (~100 KHz, Figure 6A right). Increase in the cell membrane thickness compromised ψ org , so that higher frequency was needed to induce considerable polarization in the organelle (Figure 6B left). Variation in membrane thickness did not significantly alter the phase of the organelle polarization (Figure 6B right). Since removal of the low-conductive cell membrane enhanced organelle polarization (Figure 4A), one might expect that an increase in the membrane conductivity could have a similar effect. However, within the physiological range considered in this paper, ψ org was insensitive to the cell membrane conductivity (Figure 6C left). The cell membrane conductivity did have a significant impact on the phase of mitochondria polarization. At extremely low values (<10-7S/m), ψ org demonstrated a phase advance at frequency lower than 1 KHz (Figure 6C right), rather than a phase delay, as was the case for the standard values (Figure 3C). The cell membrane dielectric permittivity represents the capacitive property of the membrane. Increase in this parameter facilitated ψ org , so that ψ org became noticeable at relatively lower frequency range (Figure 6D left). An increase in this parameter also led to a decrease in the phase delay in the organelle polarization, which was most prominent at the frequency above 100 Hz (Figure 6D right).
Dependency of ψ org on its own biophysics
Previous studies have shown that polarization of a neuronal structure depends on its own membrane properties under both electrical , and magnetic stimulations . How do the membrane properties of the organelle membrane affect its own polarization?
Similarities and differences to electrical stimulation
Analysis of ψ org under magnetic stimulation reveals several commonalities and differences to that under electric stimulation. The build up of ψ org requires the electric field to penetrate through the cytoplasmic membrane. In electric stimulation, this is achieved by directly applied electric current via electrodes. In magnetic stimulation, electric field is produced by electromagnetic induction.
Analysis on ψ org under electric field has been performed in two recent publications. Vajrala et al.  developed a three-membrane model that included the inner and our membranes of a mitochondrion, and have analytically solved ψ cell and ψ org under oscillatory electric fields. Another study  has modeled the internal membrane response to the time-varying electric field, and has investigated the condition under which ψ org can temporarily exceed ψ cell under nanosecond duration pulsed electric fields.
Results obtained from this magnetic study share several commonalities with those from AC electric stimulation. Under both stimulation conditions, ψ org can never exceed ψ cell . The ratio between the (organelle/cell) increases with frequency, and this ratio can reach 1 at very high frequency (108 Hz, data not shown). The phase responses of the organelle within a cell have not been analyzed previously under electric stimulation, which prevent direct comparison with this work. For an isolated mitochondrion, its response is similar to a single cell membrane under AC electric field stimulation , except that an extra -90° phase is introduced by electromagnetic induction (Figure 4B).
Stimulation on the internal organelle by time-varying magnetic field, though, has its own uniqueness. First, as a non-invasive method, magnetic stimulation is achieved by current induction inside the tissue, which prevents direct contact with the electrodes and introduces minimal discomfort. Second, the frequency responses of the internal organelle are different under the two stimulation protocols. In electric stimulation, magnitude of the field is independent of its frequency. In magnetic stimulation, however, the magnitude of the induced electric field is proportional to the frequency of the magnetic field (Faraday's law). Consequently, alteration in the field frequency could also contribute to ψ org . Low frequency field (< 1 KHz) is insufficient in building up noticeable ψ org and ψ cell (Figure 3A). Both ψ org and ψ cell increase with field frequency (Figure 3A). Therefore, it is unlikely possible to use high-frequency magnetic field to specifically target internal organelles, such as been done under AC electric stimulation with nanosecond pulses, for mitochondria electroporation and for the induction of mitochondria-dependent apoptosis .
Cellular factors that influence ψ cell
When a neuron is exposed to an electric field, a transmembrane potential is induced on its membrane. Attempts to analytically solve ψ cell began as early as the 1950s [51, 52]. Later works added more complexity to the modeled cell and provided insights into the factors affecting ψ cell . These include electrical properties [49, 50, 53, 54] of the cell, such as its membrane conductivity. Geometrical properties of the cell could also affect ψ cell , such as its shape [55, 56] and orientation to the field [57, 58].
Presence of neighboring cells affect ψ cell in a tissue with high-density cells, For example, isthmo-optic cells in pigeons can be excited by electrical field effect through ephaptic interaction produced by the nearby cells whose axons were activated by electric stimulation, suggesting that electrical field effect may play important roles in interneuronal communications . In infinite cell suspensions, ψ cell depended on cell volume fraction and cell arrangement . Theoretical studies have proved that presence of a single cell affected ψ cell in its neighboring cells, without direct physical contact between the two cells .
This work investigates another important factor that might affect ψ cell , i.e., presence of the internal organelle. We have previously solved ψ cell for a spherical cell model under magnetic field stimulation, without considering the presence of the internal organelle . This work extends the previous study by including an internal organelle in the cell model. Here, adding an organelle to the cell internal did not significantly change the magnitude and phase of ψ cell (Figure 5).
Factors that influence ψ org during magnetic stimulation
Biological tissue is composed of many non-homogenous, anisotropic components, such as the cellular/axonal membrane, the internal organelles and the extracellular medium. The electrical properties (i.e., conductivities) of the tissue may vary with location in the tissue, even at a microscopic level. Under magnetic stimulation, several studies have provided insights into the impact of tissue properties on field distribution and tissue polarization [42, 61].
This work further illustrates that the effects of magnetic stimulation are a function of tissue properties, by providing evidence that both the geometrical and electrical parameters of the cell/organelle membranes affect ψ org . Both the radius of the cell and the organelle strongly affect ψ org , which is in agree with previous studies [48, 62]. Radius of the neuronal structure is important in determining the threshold for its own membrane polarization, as proved by in vitro studies on eukaryotic  and bacterial cells . This model prediction is potentially testable with voltage-sensitive dyes that can provide both high temporal and high spatial resolutions [23, 65]. Another model prediction is that the amount of ψ org is insensitive to the change in cell membrane conductivity. Evidence has shown that electric field can cause long-lasting increase in passive electrical conductance of the cell membrane, probably by opening of stable conductance pores . The opening and closing of ion channels can also alter the membrane conductance. This model prediction can be tested by varying membrane conductivity, using ion-channel blockers applied to the cell membrane.
Implications for transcranial magnetic stimulation (TMS)
Another important finding in this study that within the frequency band used TMS, ψ org is insignificant comparing with ψ cell . At 10 KHz, a frequency that corresponds to the rising time of the electric pulses used in clinical TMS, the field causes considerable amount of change in ψ cell , but only 0.08 mV change in ψ org (Figure 3A). It is worth noting that even this value was probably a consequence of overestimation in the magnetic field intensity (B0). To simplify the calculation, B0 was a constant (2 Tesla) everywhere in the modeled region. In reality, the intensity of the magnetic field generated by a coil could decay quickly in the tissue far away from the coil [67, 68]. The duration of the stimulation time was also likely overestimated. During TMS, neuronal responses are induced by pulses, as opposed to the mathematically more tractable sinusoidal stimulus used in this model. Under this scenario, the magnetically-induced electric field in the tissue (essentially the change in the transmembrane potential) is determined by , which means the transmembrane potential can only be induced during the rise time (and decay time) during a step in the B field. Indeed, rise times of the field affect stimulation in clinic practice, and a faster rise time pulse is more efficient . Therefore, ψ org is unlikely significant enough in TMS to have physiological implications, and internal organelles such as mitochondria are not likely be the target in TMS practice. This conclusion is made after extensive analysis on model parameters with the values in broad physiological ranges (Table 1). To our knowledge and based on a Medline search, there have been no reports on mitochondria-related effects in TMS practice.
This paper provides two mechanisms to account for the ineffectiveness of magnetically-induced polarization in internal organelles under TMS parameters. First, the cell membrane, which is made up of lipids and proteins, provides a dominant "shielding effect" on the organelles and prevents certain amount of electric fields to penetrate into the cell membrane and polarize the organelle membrane (Figure 4). Second, the radius of the organelle is always much smaller than that of the cell, which render them relatively insensitive to the magnetic field.
Several simplifying assumptions were proposed in this model to facilitate the derivation of the analytical solutions. The model assumed that the cell was located in an electrically homogenous extracellular medium, which was an over-simplification of the true electrically anisotropic extracellular environment. Both the extracellular medium and cytoplasmic environment are not truly homogenous [69, 70]. We found that neither parameter significantly affects the organelle or cytoplasmic membrane polarization (not shown).
Both the cell membrane and the mitochondria membranes were modeled as a single spherical shell. In reality, however, cellular structures have irregular shape, which may play an important role in the dynamics of membrane polarization [71, 72]. The interior sphere was centered inside the cell to allow for mathematical simplicity of the model. However, as organelle locations vary spatially in a cell, we hypothesize that organelles located off-center of the cell or closer to the exterior cell membrane may be more sensitive to the applied field. Also, we believe the "shielding effect" of the cell membrane persists even when the separation distance between the two membranes is small (data not shown). The membrane of the organelle was modeled as a single internal shell as in a previous study , rather than a two-shell structure, representative of the inner and our membranes of a mitochondrion, respectively . The highly curved projections of the cell body and the organelle membrane may provide focal points for even greater changes in the induced transmembrane potential . Future study will use numerical methods with multi-compartment modeling or finite element meshes to represent these structure complexities.
All the dielectric permittivities in the model were assumed to be frequency-independent, which was valid for the low frequencies considered (10-200 kHz). When field frequency exceeds several hundreds of megahertz, the finite mobility of molecular dipoles starts to weaken the polarization processes . This phenomenon, known as dielectric relaxation, is characterized with decrease in the permittivities and increase in the conductivity. When this happens, the complex conductivity should be defined as S = σ (ω) + jωε (ω), where σ (ω) and ε (ω) are frequency-dependent conductivity and permittivity, respectively. By implementing this term in equations (14) and (15), one can estimate the transmembrane potentials in the cell and in the organelle when dielectric relaxation occurs.
This work provides the first analytical solution for the transmembrane potentials in an internal organelle (ψ org ) in response to time-varying magnetic stimulation. The frequency response of the membrane under magnetic stimulation is different from that under electric field stimulation. This work provides evidence that the presence of the internal organelle does not significantly affect polarization of the cell membrane (ψ cell ). Moreover, ψ org is always smaller than ψ cell under low frequency range (< 200 KHz), largely due to the "shielding effect" imposed by the presence of the cell membrane. Both the geometrical and electrical properties of the cell membrane affect ψ org in a frequency-dependent manner. The properties of the organelle membrane also affect ψ org in a frequency-dependent manner. Finally, the present study provides evidence that normal mitochondrial functionality is not likely affected by transcranial magnetic stimulation, via altering its membrane potential.
Determining unknown coefficients C n , D n in equation (13) using boundary conditions
We solved (A-11) to (A-18) the last eight unknown coefficients D0-D3, C1-C4. (see Additional file 2).
This work was supported by CIHR and a Canadian Heart and Stroke Foundation postdoctoral fellowship to Hui Ye. The authors thank Joe Hayek for valuable comments to the paper.
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