Relationship between oxygen supply and cerebral blood flow assessed by transcranial Doppler and near – infrared spectroscopy in healthy subjects during breath – holding

Background Breath – holding (BH) is a suitable method for inducing cerebral vasomotor reactivity (VMR). The assessment of VMR is of clinical importance for the early detection of risk conditions and for the follow-up of disabled patients. Transcranial Doppler ultrasonography (TCD) is used to measure cerebral blood flow velocity (CBFV) during BH, whereas near-infrared spectroscopy (NIRS) measures the concentrations of the oxygenated (O2Hb) and reduced (CO2Hb) hemoglobin. The two techniques provide circulatory and functional-related parameters. The aim of the study is the analysis of the relationship between oxygen supply and CBFV as detected by TCD and NIRS in healthy subjects performing BH. Methods 20 healthy subjects (15 males and 5 females, age 33 ± 4.5 years) underwent TCD and NIRS examination during voluntary breath – holding. VMR was quantified by means of the breath-holding index (BHI). We evaluated the BHI based on mean CBFV, O2Hb and CO2Hb concentrations, relating the baseline to post-stimulus values. To quantify VMR we also computed the slope of the linear regression line of the concentration signals during BH. From the NIRS signals we also derived the bidimensional representation of VMR, plotting the instantaneous O2Hb concentration vs the CO2Hb concentration during the BH phase. Two subjects, a 30 years old current smoker female and a 63 years old male with a ischemic stroke event at the left middle cerebral artery, were tested as case studies. Results The BHI for the CBFV was equal to 1.28 ± 0.71 %/s, the BHI for the O2Hb to 0.055 ± 0.037 μmol/l/s and the BHI for CO2Hb to 0.0006 ± 0.0019 μmol/l/s, the O2Hb slope was equal to 0.15 ± 0.09 μmol/l/s and the CO2Hb slope to 0.09 ± 0.04 μmol/l/s. There was a positive correlation between the CBFV and the O2Hb increments during BH (r = 0.865). The bidimensional VMR pattern shows common features among healthy subjects that are lost in the control studies. Conclusion We show that healthy subjects present a common VMR pattern when counteracting cerebral blood flow perturbations induced by voluntary BH. The proposed methodology allows for the monitoring of changes in the VMR pattern, hence it could be used for assessing the efficacy of neurorehabilitation protocols.


Background
Unlike the other organs, human brain needs a constant oxygen supply in order to maintain its functional and structural integrity. The local amount of oxygen stored in the brain tissues is small compared to the metabolic needs, hence a specific mechanism is necessary in order to ensure the correct oxygenation levels. This mechanism has to provide oxygen during both resting condition and focal cortical activity. The strict coupling existing between "activation", local oxygen consumption, and increased regional cerebral blood flow constitutes the basis of the so called BOLD effect (Blood Oxygenation Level Dependent) and, hence, of the functional magnetic resonance [1]. Thus, the assessment of cerebral hemodynamics is of paramount importance for determining the response of a subject to an external stimulus or for quantifying cortical activation.
Among the methods allowing a non -invasive and lowcost assessment of cerebral hemodynamics, transcranial Doppler ultrasonography (TCD) plays a fundamental role [2,3]. By means of TCD it is possible to measure the cerebral arteries blood flow velocity (CBFV) and, hence, analyze the variation of the CBF. However, the limited spatial resolution of this technique allows for the quantification of CBFV only in the macro -vessels (essentially the arteries constituting the Willis circle plus the middle cerebral arteries), whereas a cortical localized modification of blood velocity is impossible to track. Moreover, in about 25% of the patients, it is impossible to perform a TCD examination due to poor skull acoustic windows.
By means of near -infrared spectroscopy (NIRS) it is possible to continuously monitor the local concentrations of oxygenated (O 2 Hb) and reduced (CO 2 Hb) in the adult brain. TCD provides a direct measurement of circulatory parameters, whereas NIRS provides more functional and activation-dependent informations. Specifically, it has been demonstrated that NIRS can proficiently measure cerebrovascular reactivity [4].
In clinical practice, cerebral autoregulation is usually assessed during a CO 2 reactivity test [5]. It is known that baroreceptors react to an increased partial pressure of CO 2 by inducing vasodilatation in the resistance vessels; hence, the mean CBFV increases and the resistance of the vessels drops [6]. This mechanism is often indicated as vasomotor reactivity (VMR). CO 2 reactivity can be induced by means of acetazolamide injection, by means of direct CO 2 inhalation (usually at the 5% -7% concentration), or by means of simple breath -holding (BH).
In the last five years, a great variety of studies combining TCD and/or NIRS have been devoted to the assessment of VMR in subjects affected by acute and chronic patholo-gies: microangiopathy [7], migraine [8], carotid artery occlusion [9] and depression [10]. Recently, NIRS has been also used for the cerebral activity quantification during motion tasks [11]. From a rehabilitation point of view, NIRS proved successful in monitoring motor reorganization in hemiparetic stroke patients [12].
Traditionally, in response to a CO 2 test, VMR is quantified by relating baseline values (these values can be the mean CBFV as well as the concentrations of O 2 Hb and CO 2 Hb) to post -stimulus values [9]; while the stimulus phase is not taken into consideration. Since VMR determines a continuous modification of such values during time, omitting the analysis of the stimulus phase may lead to uncertainties and poor comprehension of the VMR itself.
The aim of the study is the analysis of the relationship between oxygen supply and CBFV as detected by TCD and NIRS in healthy subjects performing BH. We studied a population consisting of 20 healthy volunteers and we showed the vasoreactivity patterns the subjects had during BH. We introduced a bidimensional representation of VMR based on the O 2 Hb and CO 2 Hb concentration changes that we consider useful to gain a better comprehension of VMR. Finally, we showed that this methodology could be used for assessing a subject's VMR condition, comparing the data of two case studies to those of the normal population.

Subjects
Currently, we enrolled in this study 20 (15 males and 5 females) healthy non-smokers volunteers (age, mean ± sd = 33 ± 4.5 years). Before being included in this study, all the subjects underwent clinical examinations intended to exclude cerebral, cardiac, and circulatory diseases. According to the rules of the local Hospital in which the tests were hold, the subjects were asked to sign an informed consent.

Case studies
We also tested several healthy current smokers subjects and some pathologic subjects. Due to the great variability of our sample population of smokers and pathologic subjects, we decided to present in this paper only two case reports which we found indicative of their category. The first subject was a healthy current smoker 30 years old female. She had been smoking for 12 years and she smoked an average of 15 cigarettes/day. The subject (indicated as subject A in the following) underwent the same clinical examinations of the normal controls and did not show any sign of cerebral, cardiac, and circulatory diseases. The second subject was a post-stroke, 63 years old, man. He had suffered from a ischemic stroke to the left middle cerebral artery (MCA) about 2 years before being enrolled in the study, when he was tested for the first time. He showed aphasia, motor impairment, and poor scores in fluency and verbal tests. After a year of drug therapy (antihypertensive and antiaggregating agents) and logopedic therapy, this subject was tested for the second time. He reported an improvement in motor control and reaching tasks, and increased his AAT (Aachener Aphasie Test) score from 52/60 to 56/60.

Measurement protocol
We applied TCD and NIRS during baseline conditions and during CO 2 reactivity. To trigger CO 2 reactivity, we chose the voluntary breath -holding technique. A major advantage of this choice is simplicity, since, to induce hypercapnia, there is no need for further devices (i.e. a capnograph with a breathing mask). This technique, however, is subject dependent: it is impossible, in experimental conditions, to establish a BH duration equal for all the subjects. To cope with this difficulty, we preliminary instructed the subjects on how to perform the BH and we let them test the procedure once before starting the recordings. In particular, we instructed the subjects to hold the breath after a normal breathing, in order to avoid an increase of the thoracic pressure, and we controlled they could hold the breath for a minimum time of 20 s. According to previously published experimental protocols, we instructed the subjects to end breath -holding when they felt comfortable [13].
The experimental protocol was the following: • to derive baseline conditions, the subjects were allowed to rest for about 10 minutes in a dimmed and quiet room, laying comfortably in a supine position with eyes closed and breathing room air; • when we observed stable signals (i.e. when the concentrations of O 2 Hb and CO 2 Hb and the CBFV did not show remarkable variations from their mean values), the subjects were instructed to perform a breath -holding after a normal inspiration; • at the end of the apnea, the subjects were asked to rest for 5 minutes and we collected signals related to the post -stimulus conditions.

TCD recordings
We recorded the CBFV in both the middle cerebral arteries simultaneously by means of a commercially available transcranial Doppler device (Multidop X4, DWL, Germany) equipped with 2 MHz probes. The transducers were positioned in order to insonate the MCAs in their Ml tract by the temporal bone windows. Probes positioning and the obtained Doppler sounds were confirmed on the basis of currently adopted clinical standards [14]. As an example, figure 1 depicts the modifications of the left MCA CBFV of a healthy subject performing BH. The figure reports the envelopes of the Doppler spectrum in function of time. It can be noticed how CBFV progressively and almost linearly increases while BH is maintained and then quickly recovers baseline conditions after breath release.

NIRS recordings
Changes in the concentrations of O 2 Hb and CO 2 Hb were measured by means of a near -infrared spectroscopy device (NIRO 300, Hammamatsu Photonics, Australia). The emitting probe of the NIRS equipment was placed on the left frontal side of the subjects, 2 cm beside the midline and about 3 cm above the supraorbital ridge. We chose this positioning in order to avoid the sinuses and to place the probes on a poorly perfused and very thin skin layer. BH is supposed to induce a perturbation in cerebral cortex that is systemic and not regional or localized, hence the frontal lobe was a suitable location also for the absence of hairs. The receiving sensor was fixed laterally to the emitter at a distance of about 5 cm. According to previous studies and theoretical models already developed [15], we set a differential pathlength factor equal to 5.97. Previous works [15,16] demonstrated that with a sourcedetector distance equal to approximately 5 cm the NIRS equipment is capable of detecting effectively the chromophores concentration changes on the surface of the cerebral cortex.  Chromophores concentration changes were acquired continuously at a sampling rate equal to 2 Hz. To avoid bias from environmental light, a black cloth covered the NIRS probe. As an example, figure 2 reports the time course of the two types of hemoglobin during BH.

CBFV modifications during BH of a healthy subject
During the test, we also monitored the end-tidal CO 2 and the mean arterial blood pressure by means of a specific monitor equipped with a capnographic module.

Vasoreactivity quantification
According to previous studies [8], we used the breathholding index (BHI) to quantify vascular reactivity. This index can be defined for any quantity related to the cerebral circulation, since it simply relates post -stimulus quantities to pre-stimulus quantities.
From the TCD data, we derived a BHI based on the mean blood flow velocity (MV). MV can approximately be defined as [17]: where: • PV is the peak systolic blood flow velocity; • EDV is the end -diastolic blood flow velocity. Figure 3 sketches the meaning of the PV, EDV, and MV in relation to the envelope of the CBFV during two cardiac cycles.
The BHI derived from the MV (which is indicated as BHI V in the following) was then defined according to the following expression: where: • V BASE represents the MV averaged on a 10s time window when in baseline conditions; • V BH represents the MV averaged on a 10s time window after the offset of the apnea; • D BH is the time duration of the BH.
This index is expressed in %/s. From the TCD data, we also calculated the Gosling's pulsatility index (PI) of the MCA in baseline conditions and in correspondence of the maximum CBFV increase during the apnea. The PI is defined according to the following expression: This parameter indicates how the ratio between the extreme velocities in the artery modifies as consequence of vasoreactivity and it is often used in VMR studies as a complement to the BHI [2]. To quantify VMR from the NIRS data, we estimated the chromophores concentration changes with respect to the BH duration [7]: As in equation 2, O 2 Hb BASE is the oxygenated hemoglobin concentration in baseline conditions, averaged on the same 10s time window during which the V BASE is evaluated, and O 2 Hb BH is the average concentration after the release of the BH. We calculated the same index also for the CO 2 Hb ( ) .

MV PV EDV
Hb and CO 2 Hb concentration changes during BH of a healthy subject

VMR bidimensional representation
To obtain the VMR bidimensional pattern during BH, we lowpass filtered the O 2 Hb and CO 2 Hb concentration signals (15 order Chebyshev digital filter, with ripple in the stop band, cutoff frequency equal to 50 mHz and at least 30 dB of discrimination) and set the initial concentrations equal to zero. The O 2 Hb and CO 2 Hb concentration signals were then normalized with respect to their maximum value during the BH phase. Then, in a bidimensional plane, for each time instant, we plotted the O 2 Hb vs the CO 2 Hb concentration. Lowpass filtering was introduced to obtain smooth profiles in the bidimensional representation; the zero setting of the initial conditions ensured that all the bidimensional patterns started form the graph origin, hence were direclty comparable. The resulting bidimensional plot are reported by figure 6.

Carbon dioxide reactivity triggered by breath -holding
As already pointed out, the three major techniques adopted for triggering CO 2 reactivity are: hypercapnia, acetazolamide injection, and breath -holding [5]. We decided to carry on this study using BH as reactivity trigger, since we planned to develop an experimental protocol that could be suitable for any subject, including patients suffering from cerebrovascular, neurological, and chronic diseases.
Breath -holding is obviously subject dependent; while this poses the problem of dealing with different BH durations, we believe this technique is suitable for assessing VMR as response to a sudden and abrupt change in the oxygenation levels, which is a major risk condition for cerebral autoregulation.

VMR quantification
The population averaged BH duration was 41.7s ± 8.3s (95% confidence interval ranging from 38.1s to 45.4s). Table 1 reports the BHI V and the PI values derived from TCD measurements of the CBFV. The average increase in the CBFV was equal to 1.28 %/s ± 0.71 %/s, whereas the PI decrease from an initial average value equal to 0.86 to Evaluation of the slope of the chromophore concentration changes Figure 4 Evaluation of the slope of the chromophore concentration changes. Sketch of the slope computation for the O 2 Hb concentration signal of a healthy subject during BH: from the minimum and the maximum point of the concentration during BH, the angular coefficient of the linear regression line is computed. This slope is taken as index of VMR.   [17]. From a methodological point of view, the neat decrement of the PI confirms that the experimental protocol was suitable for triggering vasomotor reactivity: during BH, the EDV increase was greater than the PV increase, hence PI diminished. Usually, the decrement of the PI is used to confirm the drop in the periferal vessel resistance, hence to ensure a correct onset of VMR. We believe that the quantification of VMR by means of the BHIs derived by NIRS signals could be questioned. According to literature, vasomotor reactivity is quantified as the variation of a given physiological parameter as consequence of an external stimulus (usually a CO 2 increase). As a matter of fact, however, the above defined indices only depends on the baseline and on the post-BH conditions, but what happens during the BH phase is not taken into consideration.
Mean CBFV increases during CO 2 reactivity tests as consequence of a pial arteries vasodilation, but then it remains almost constant for periods lasting several seconds [2]. Hence, the quantification of vasomotor reactivity based on pre-apnea and post-apnea values is appropriate. Conversely, as our experimental results clearly show, the local concentration of oxygenated hemoglobin measured by

O 2 Hb CO 2 Hb
NIRS is a more rapidly evolving quantity, since it depends on the CBFV, on the perfusion pressure, on the degree of artery dilation and on the tissues oxygen extraction rate. Moreover, vasoreactivity is triggered by a CO 2 increase, but the quantification of VMR itself is usually done by taking into account the increases in both oxygenated and reduced hemoglobin; this because VMR is a functional physiological process aiming at maintaining a proper chromophores concentration in brain tissues. Hence, we believe that for a proper interpretation and evaluation of the VMR during BH it is necessary to observe the reactivity pattern during the apnea phase. We propose to measure the slopes of the O 2 Hb and of the CO 2 Hb concentration signals and to use them for quantifying VMR during voluntary breath-holding. This quantity, in fact, is strictly related to the time course of the hemoglobin concentration signal. This index is also implicitly normalized with respect to the BH duration; this enables direct a comparison of the results among different subjects.
Our results also revealed a good correlation between the BHI V and the slopes of the O 2 Hb and of the CO 2 Hb concentration signals: figure 7 reports the scatter diagrams of the BHI V and of the slopes (O 2 Hb on the left panel and which is greater than that of CO 2 Hb, has a greater correlation with the increase in CBFV; this is not surprising since O 2 Hb concentration is predominant in the cerebral cortex. Approximating the increase of the regional cerebral blood volume with the O 2 Hb concentration increase, in healthy subjects performing our experimental protocol, an increase in CBFV is almost linearly correlated with the increase of the local cerebral blood volume.  slope CO 2 Bidimensional VMR representation derived by NIRS signals Figure 6 Bidimensional VMR representation derived by NIRS signals. Bidimensional VMR patterns as assessed by NIRS signals for the sample population. Each red circle represents the instantaneous concentration of CO 2 Hb (horizontal axis) and O 2 Hb (vertical axis). The concentration values are normalized with respect to their maximum value during the BH phase. The dotted lines depict the first and third quadrants bisectors. The reactivity pattern is always comprised into the region delimited by the two bisectors, evidencing a greater increase in the O 2 Hb level with respect to the CO 2 Hb concentration level. can be observed on the time course of the two concentrations:

NIRS vasoreactivity patterns
1. an initial phase, similar to the the baseline, in which the two chromophores concentrations do not significantly change; 2. the VMR phase, in which there is a strong increase of the O 2 Hb (and, hence, of the total hemoglobin, that roughly corresponds to the regional cerebral blood volume) while the CO 2 Hb is kept at a baseline level; 3. a plateau phase when the vasodilation has already reached its maximum, characterized by an almost constant level of O 2 Hb and a progressive increase of the CO 2 Hb level.
At the end of the BH, a recovery phase takes the concentration signals to baseline values. Despite the great variability affecting the NIRS signals, we found these common features in all the subjects we tested, provided that the BH duration was at least of 20 seconds. Figure 5 reports the population averaged O 2 Hb (left diagram) and CO 2 Hb (right diagram) concentration signals during BH. In order to make the signals comparable, we normalized the BH duration of each subject and set the initial concentrations (i.e., at the BH onset) equal to zero. The superimposed vertical bars represent the instantaneous standard error. Starting from 20% of the BH duration, the O 2 Hb signal depicts an increase in the variability that is due to the fact that, by that time, VMR had its onset. The linear increase of the O 2 Hb continues until 80% of the BH duration, then variability reduces and a region of plateau can be observed. Conversely, the CO 2 Hb shows a more variable behavior, but its average concentration remains at baseline values almost until the 90% the BH, when an increase, which cannot be further compensated, determines the end of the BH.

Bidimensional VMR representation
Vasoreactivity is a physiological mechanism that ensures the correct brain oxygenation both in baseline conditions and dynamically in consequence of perturbations to the blood oxygenation level. Specifically, during hypoxaemia, the decrease of the arterial partial pressure of oxygen, and the consequent increase of the arterial partial pressure of carbon dioxide, triggers VMR. The mechanisms that determine the onset of vasoreactivity are still debated [18].
If TCD is useful to document the increased CBFV as a physiological response to an increased oxygen demand by the brain tissue and to estimate the drop of the pial arteries resistance, NIRS could be proficiently used to monitor VMR in relation to the local amount of oxygen consump- slope O 2 slope CO 2 tion and extraction. To this purpose, we propose to observe the VMR pattern in a two-dimensional plane, where it is possible to monitor the instantaneous balancing of the two types of hemoglobin and to determine how autoregulation varies the concentration of the two chromophores. Figure 6 reports the bidimensional BH patterns as assessed by means of the NIRS signals. The horizontal axis reports the instantaneous concentration of CO 2 Hb (normalized with respect to its maximum value during BH), whereas the vertical axis reports the O 2 Hb one (normalized with respect to its maximum value during BH). The dotted lines represent the first and third quadrant bisectors: when the VMR pattern is in the region comprised between the two bisectors, it means that the oxygenated hemoglobin concentration is increasing and, more specifically, it is increasing more than the reduced hemoglobin concentration. It is possible to notice that the VMR pattern is always comprised into this region. An initial increase in the CO 2 Hb concentration is rapidly compensated by a steep increase in the O 2 Hb concentration. Contemporarly, Bidimensional VMR pattern for 4 healthy subjects Figure 8 Bidimensional VMR pattern for 4 healthy subjects. Bidimensional reactivity pattern as derived by the NIRS signals for four healthy subjects. Each red circle represents the instantaneous concentration of CO 2 Hb (horizontal axis) and O 2 Hb (vertical axis). All the values are normalized with respect to the maximum. The dotted lines depict the first and third quadrants bisectors. All the graphs present characteristics of the VMR pattern of healthy subjects and are almost always comprises into the region delimited by the two bisectors. 15 subjects showed patterns similar to A and B, 4 subjects showed a pattern similar to graph C, whereas graph D is relative to the subject that showed the shorter plateau region. C D CO 2 Hb is kept at a concentration a little lower than the initial one. When the vasodilation has reached its maximum, there's a plateau region in which the O 2 Hb concentration remains almost constant, while the CO 2 Hb concentration starts increasing; afterwards, BH ends. This behavior was found for all the healthy subjects tested: figure 8 depicts the bidimensional VMR pattern for four different subjects. Even though the four patterns are different, there are common features that are characteristic of an intact autoregulation mechanism: i) after a very short initial phase, the VMR pattern is always comprised into the region delimited by the first and third quadrant bisectors; ii) CO 2 Hb is kept at baseline concentrations during the VMR phase, or, in some subjects, may decrease its concentration (graph C); iii) the final portion of the BH is characterized by a plateau region during which O 2 Hb is almost constant and CO 2 Hb tends to increase (a brief plateau region is observable in graph D, this pattern is relative to the subject that showed the minimum and shorter plateau phase).

Correlation between BHI V and slopes of the hemoglobin signals
A validation of these result is not straightforward: there are no studies, that we are aware of, that derived such bidimensional patterns from NIRS signals. However, the highly repeatable pattern we found in normal subjects suggests that cerebral autoregulation shows common features when counteracting the effects of BH. From a methodological point of view, we believe that the observation of the bidimensional pattern may be of help in interpreting more complex practical situations where autoregulation is impaired: in these conditions, a different balancing of the two chromophore concentrations could be expected. The following section reports two case studies, whose TCD and NIRS data are compared to our normative data.

Case reports Subject A -current smoker
This subject could voluntary hold the breath for 24 seconds, hence significantly less than the average of the normal controls. The first row of Table 3   Moreover, BH ends without reaching a plateau condition. The right panel of figure 9 shows the bidimensional VMR pattern derived by the NIRS data. It is evident that vasoreactivity is different from the pattern of normal subjects: the VMR pattern constantly moves in the 2D plane towards the increasing CO 2 Hb concentration direction and the increase in the O 2 Hb concentration is insufficient.
As a consequence, the VMR pattern is never comprises between the two bisectors. Breath -holding, also, ends without reaching a plateau phase, hence it is impossible to state if this subject could compensate by reaching his maximum vasodilation. Several studies have already been devoted to the quantification of VMR in healthy current smokers (see [19,20] among others), even though results are not always in accordance each other: if some authors found a reduced cerebral blood volume during hypercapnia [21,22], other investigators did not find repeatable VMR patterns [23]. By means of our technique, we could document the delayed onset of VMR, the uncompensated CO 2 Hb concentration rise during BH, the VMR bidimensional pattern always out of the bisectors region, and the absence of a plateau region, that could stand for a chronic alteration of current smoking on the baroreceptor control [24].

Subject B -post-stroke subject
During the first test, this subject could hold the breath for 47 seconds. Despite the good duration of BH, the second row of Table 3 reveals how VMR was strongly impaired: the BHI V was very small, and there was no drop of resistance in the peripheral vessels due to apnea (PI greater after BH than in baseline conditions). NIRS data confirmed this absence of VMR: , , and were extremely low. Figure 10 (left panel)  figure 10 depicts the bidimensional VMR pattern and confirms the absence of vasoreactivity: the hemoglobin concentrations change with no functionally significant coordination. Clinically, this subject suffered form an ischemic event to the left MCA, which determined a peripheral vasodilation and the onset of a compensatory circulation in the other branches of the Willis' circle. Hence, this subject was unable to react to a carbon dioxide increase since, to counteract the effects of stroke, its arteriolar bed was already in vasodilation conditions.
After being treated with drugs and logopedic therapy for one year, the subject improved his motor and phasic performances. The results of the BH test reveal the effects of the therapy: the BHI V increases and the PI shows a drop during BH, meaning a little vasodilation is now present. Also, , and increased, demonstrating that the subjects improved its reaction to the apnea. Figure 11  Even though further studies are required, we believe this analysis methodology could be useful for monitoring and quantifying the effects of neurorehabilitation trials.

Conclusion
In this paper we proposed a methodology for the assessment of VMR during voluntary BH. This methodology relates oxygen supply to cerebral blood flow by calculating BHIs based on TCD and NIRS data. We introduced a bidimensional representation of VMR during BH that we consider important to monitor the unbalancing between O 2 Hb and CO 2 Hb as consequence to a varied local oxygen demand.
On a population of 20 healthy subjects, we showed that the increment of the cerebral blood flow velocity in the middle cerebral artery is linearly correlated to the increment of the O 2 Hb when vasoreactivity is triggered by voluntary breath holding. Moreover, we provided normative BHI values on this sample population.
We observed that the vasoreactivity pattern of healthy subjects is characterized by common features that are not present if autoregulation is impaired: as an example we presented two case studies (a current smoker healthy sub-