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Feasibility and effects of patient-cooperative robot-aided gait training applied in a 4-week pilot trial



Functional training is becoming the state-of-the-art therapy approach for rehabilitation of individuals after stroke and spinal cord injury. Robot-aided treadmill training reduces personnel effort, especially when treating severely affected patients. Improving rehabilitation robots towards more patient-cooperative behavior may further increase the effects of robot-aided training. This pilot study aims at investigating the feasibility of applying patient-cooperative robot-aided gait rehabilitation to stroke and incomplete spinal cord injury during a therapy period of four weeks. Short-term effects within one training session as well as the effects of the training on walking function are evaluated.


Two individuals with chronic incomplete spinal cord injury and two with chronic stroke trained with the Lokomat gait rehabilitation robot which was operated in a new, patient-cooperative mode for a period of four weeks with four training sessions of 45 min per week. At baseline, after two and after four weeks, walking function was assessed with the ten meter walking test. Additionally, muscle activity of the major leg muscles, heart rate and the Borg scale were measured under different walking conditions including a non-cooperative position control mode to investigate the short-term effects of patient-cooperative versus non-cooperative robot-aided gait training.


Patient-cooperative robot-aided gait training was tolerated well by all subjects and performed without difficulties. The subjects trained more actively and with more physiological muscle activity than in a non-cooperative position-control mode. One subject showed a significant and relevant increase of gait speed after the therapy, the three remaining subjects did not show significant changes.


Patient-cooperative robot-aided gait training is feasible in clinical practice and overcomes the main points of criticism against robot-aided gait training: It enables patients to train in an active, variable and more natural way. The limited number of subjects in this pilot trial does not permit valid conclusions on the effect of patient-cooperative robot-aided gait training on walking function. A large, possibly multi-center randomized controlled clinical trial is required to shed more light on this question.


Patients with motor dysfunction due to lesions of their central nervous system (CNS) typically undergo physical and occupational therapy for rehabilitation. In the past, this therapy mainly consisted of stretching, bracing and strengthening of the affected limbs as well as training of compensation strategies using unaffected limbs to allow patients to become as functional and independent as possible [1]. Since research on neural plasticity has started to stress the ability of the CNS to reorganize and relearn, therapy approaches have emerged that focus on exploiting this plasticity in a functionally beneficial way. A prominent approach in this direction has been pioneered by the American psychologist Edward Taub. Based on the psychological concept of "learned helplessness" [2], he argued that especially hemiplegic patients may end up in a state of "learned non-use" of their paretic limbs if they are not sufficiently encouraged to use them. This (psychological) state would then also be unfavorable in the perspective of neural plasticity, as the CNS is not driven to reorganize itself in support of the affected limbs. To prevent these negative developments, Taub and his colleages designed the technique of constraint-induced movement therapy (CIMT), also referred to as "forced use" or "constrained-induced therapy" [3], which has shown to be effective in randomized controlled trials [46]. Furthermore, animal studies are starting to shed light on the underlying physiological mechanisms of recovery induced by CIMT [7].

Body-weight supported treadmill training

In the light of neural plasticity and basic principles of motor learning it seems apparent that rehabilitation training should be task specific, i.e. if the aim is to relearn walking, one should practice walking. This common-sense argument for task specificity has been demonstrated to be valid by a large body of research, e.g. [812].

To perform task-specific gait training in a safe environment, body-weight supported treadmill training (BWSTT) has been introduced [13, 14]. In this approach, patients wear a harness and are partially relieved from their body weight by a body-weight support system. BWSTT has become a well-accepted task-specific therapy to retrain gait in individuals with neurologically caused walking impairments. While BWSTT does not appear to be more effective than other task-specific approaches such as overground mobility training [15, 16], it causes better results than unspecific lower extremity strength training [17].

Robot-assisted gait training

For severely affected patients, task-specific training for walking—be it on a treadmill or overground—puts a substantial physical burden on therapists, who not only have to facilitate the desired movements of paretic and potentially spastic limbs but also need to guarantee the safety of their patients and prevent them from falling. Therefore, even with the additional safety of the body-weight support system in BWSTT, two or three therapists are needed to train a para- or tetraplegic individual.

These limitations motivated interdisciplinary teams of engineers and clinicians to develop technical tools that reduce the burden of manually assisted gait training. In the late 90s of the 20th century, the Lokomat [18, 19] and the gait trainer GT1 [20] were developed and subsequently commercialized.

Both devices became commercially successful and were soon followed by others: The AutoAmbulator/ReoAmbulator (Motorika) [21], and the Haptic Walker [22], which has been commercialized as G-EO (RehaTechnologiesa).

In addition to the commercially available gait rehabilitation robots, countless research prototypes have been developed, e.g. [2326].

Clinical research about the efficacy of robot-aided gait training is still at an early, rather inconclusive state [27, 28]. For the Lokomat, studies with stronger focus on non-ambulatory subjects found advantages of robot-aided gait training over manually assisted gait training [2932], while studies focusing on ambulatory subjects found manually assisted gait training to be more effective [33, 34]. These results suggest that currently, robot-aided treadmill training is most effective for severely affected, non-ambulatory patients, whereas it may not be ideal for more advanced, ambulatory patients. This situation demonstrates the need to improve current rehabilitation robots in a way that extends their spectrum of effective treatment to functionally more advanced patients. Such an improvement would allow patients to benefit from robot-aided treadmill training throughout their different stages of recovery, up to a point where they can safely and efficiently perform overground training.

Furthermore, the repetitive gait pattern imposed by a position-controlled robot may not provide sufficient variability to drive the reorganization of the CNS in an optimal way. Variability in the input data is required for neural networks to improve fault-tolerance, generalization, and learning [35]. In training theory, this observation has been well-known for a long time, and it has been captured nicely in the phrase coined by Bernstein that training should be "repetition without repetition" [36].

Previous work

The first controllers for robotic devices supporting gait training were position controllers with the aim to ensure that the robot (and the patient) followed the desired movement trajectory as closely as possible [18, 20]. These controllers were essentially "blind" to the actions of patients, i.e. active movements in the desired direction were equally prohibited as active movements in other directions. Thus, they did not encourage patients to participate actively in the training and reduced movement variability to a minimum.

Efforts were then taken to introduce more compliant control schemes, particularly impedance control[37]. Here, the current position of the robot is virtually coupled to a reference position by a simulated spring and damper assembly with adjustable stiffness and damping values. The spring and damper action is emulated by motors, which apply forces on the patient. With reduced spring stiffness, patients can participate more actively and experience more movement variability [38]. However, they can also lead to unfavorable movement patterns and become more and more affected by the inertia of the robots as impedance is reduced.

The assist-as-needed (AAN) paradigm[39, 40] has been introduced to generally improve the behavior of rehabilitation robots. It states that a rehabilitation robot should constantly try to reduce its support so that patients receive just the minimum of support required to overcome their weakness or impaired motor control. Then, patients would not be able to "slack" and rely on the support of the robot, and the danger of "learned helplessness" would be avoided.

Besides these general trends, there have been many efforts to develop controllers aiming to improve robot-aided gait rehabilitation in recent years [24, 39, 4148].

One particular approach, motivated by the experiments performed in animal experiments by Cai and colleagues [11, 49], is the patient-cooperative Path Control strategy, which we implemented for the Lokomat gait rehabilitation robot. The control strategy allows patients to influence the timing of their leg movements along the spatial path of a physiologically meaningful walking pattern. The robot simulates compliant virtual walls, which keep the patient’s legs within a "tunnel" around the desired spatial path [50].

We have previously investigated the immediate effects of the Path Control strategy versus non-cooperative robot-aided gait training on individuals with incomplete spinal cord injury (iSCI). Eleven individuals with iSCI participated in a single training session with the Lokomat. The participants trained more actively and with larger kinematic variability while the Lokomat was controlled with the patient-cooperative Path Control strategy than during standard position-controlled robot-aided gait training [51].

Research questions

Based on the encouraging results of the single training session with iSCI subjects, we were interested in the feasibility and potential effects of applying the patient-cooperative training approach over several weeks. Therefore, we applied this kind of training in a pilot trial on a single case basis.

The aim of this pilot trial was to answer the following research questions:

  1. 1.

    Is it feasible to apply patient-cooperative robot-aided gait training over the course of a typical rehabilitation program of four weeks?

  2. 2.

    What is the short-term effect of patient-cooperative vs. non-cooperative robot-aided gait training on patients?

  3. 3.

    What is the effect of four weeks of patient-cooperative robot-aided gait training on walking function?


Rehabilitation device

In this study, patient-cooperative robot-aided gait training was implemented with the Lokomat gait rehabilitation robot (Figure 1). More detailed information about the device can be found in the related publications [18, 19]. Briefly summarized, the Lokomat comprises two actuated leg orthoses that are attached to the patient’s legs. Each orthosis has one linear drive in the hip joint and one in the knee joint to induce flexion and extension movements of hip and knee in the sagittal plane. Knee and hip joint torques can be determined from force sensors between actuators and orthosis. Passive foot lifters can be added to induce ankle dorsiflexion during swing phase. A body weight support system relieves patients from a definable amount of their body weight via a harness [52].

Figure 1
figure 1

The Lokomat gait rehabilitation robot (Photo courtesy of Hocoma AG).

Robotic control strategies

The main control strategy used in this trial was Generalized Elastic Path Control as introduced in [53]. In this approach, a "virtual tunnel" for the leg movements is represented by a virtual force field, represented by a mapping from the current position to the force reponse from the virtual elastic tunnel walls. The force field was obtained by an optimization algorithm, under the constraint that the field has to be conservative to ensure controller stability. Details of the procedure are reported in [53, 54]. In earlier studies, the "virtual tunnel" had been implemented by means of a nearest-neighbor search with respect to a reference trajectory [50, 51]. The transition from this earlier approach to the Generalized Elastic Path Control approach was made because the latter provided better control performance with more natural movements within the "virtual tunnel" [53, 54].

We implemented three different conservative force fields: (1) a narrow tunnel with tight coupling between the joints, (2) a tunnel of medium width, and (3) a wide tunnel, which provided only very loose coupling. The therapist was able to continuously adjust the tunnel width between these settings, causing the control algorithm to linearly interpolate between the different force fields.

We combined the Generalized Elastic Path Control approach with a standard impedance controller [38], because it was easier for patients to start the training with the reference timing provided by this strategy (Figure 2). The stiffness of this superimposed secondary controller was continuously adjustable between zero and the maximally achievable stiffness. The maximal stiffness will also be referred to as 100% guidance force, and zero stiffness as 0% guidance force. At 0% guidance force, only the virtual tunnel (and optional supportive flow) rendered by the Generalized Elastic Path Control algorithm affected the patient.

Figure 2
figure 2

Control scheme of the Generalized Elastic Path Control combined with the conventional impedance control approach. The "Path Force Field" block constitutes a conservative force field and computes the torques τelast as a function of the actual joint angles qact[53]. The corrective torques τcor of the "Impedance" block simulate a viscoelastic coupling of the actual angles qact to the reference angles qref. The "torque-controlled drives" apply the desired torques to the Lokomat orthosis ("Orthosis"). These torques are measured as τsens. Together with the interaction torques τint, they move the exoskeleton and determine the actual angles qact.

For more advanced patients, we included the possibility to influence the treadmill speed by combining the patient-cooperative Lokomat controller with the Automatic Treadmill Speed Adaptation algorithm [55]. In this approach, the horizontal ground reaction forces between the patient’s feet and the treadmill are used to intuitively control the treadmill speed during robot-aided gait training. The maximal treadmill speed for this trial was limited to 4.0 km/h (1.1 m/s).

The body-weight support system was controlled as described in [52], to provide a constant level of body-weight support set by the therapist.

A similar approach as described in [50] was used for visual feedback: An avatar representing the patient was shown with overlaid "ghost legs" which demonstrated the desired movements. Additionally, a second manikin, which walked to the left of the patient avatar, was introduced. Like the "ghost legs", this second manikin showed the desired leg movements based on the original gait trajectory enclosed by the "virtual tunnel" rendered by the Generalized Elastic Path Control, with a timing appropriate for the selected treadmill speed. The patient was instructed to try to match the movements of the red manikin (Figure 3).

Figure 3
figure 3

Visual feedback for the pilot trial. The yellow legs of the patient avatar (figure on the right) represent the actual movements of the Lokomat legs. The red figure on the left demonstrates the desired leg movements. Subjects are instructed to match the movements of the red figure as close as possible. For fine-tuning of the movements, subjects can focus on the semi-transparent red "ghost legs" that are overlaid to the legs of the patient avatar.

During training in Automatic Treadmill Speed Adaptation mode, the visual feedback was modified. The "ghost legs" were removed from the patient avatar, and the red manikin walked with a definable speed in the virtual environment. The speed of the patient avatar was coupled to the current treadmill speed, and the patient was instructed to match the speed of the red manikin. By employing a pseudo-random profile of desired speeds for the red manikin, the therapist was able to trigger patients to autonomously vary their walking speed.

Trial location and subjects

The trial was conducted at the spinal cord injury (SCI) research lab of the Spinal Cord Injury Center at Balgrist University Hospital, Zurich, Switzerland. The study protocol was approved by the Ethics Committee of the Canton of Zurich, Switzerland, and all subjects gave written informed consent prior to the experiments.

To be considered for inclusion, iSCI subjects had to be between the age of 18 and 70 with a chronic incomplete spinal cord injury (time after injury greater than 12 months). Subjects had to be rated as ASIA C or D on the American Spinal Cord Injury Association Impairment Scale (AIS) with a motor level of lesion between C4 and Th11 [56]. Furthermore, they had to be unable to walk without at least moderate assistance at the time of inclusion (i.e. a score of less than six in the "mobility outdoors" item of the latest version of the Spinal Cord Independence Measure (SCIM III) [57] was required). Cognitive capacity to follow simple verbal instructions was necessary.

For stroke subjects, the inclusion criteria were: age between 18 and 70 years, a hemiparesis which had persisted for more than 6 months after one (but not more than one) unilateral, supratentorial, ischemic or hemorrhagic stroke without bilateral, brain stem or cerebellar lesions. The lesion had to be confirmed by radiologic findings. Subjects had to be able to ambulate more than 10 m overground without assistance of a therapist at speeds between 0.1 and 0.8 m/s, using assistive devices or braces as needed. Stroke subjects had to have at least one key muscle of the affected leg with values 3 or lower according to manual muscle testing [58, 59]. Cognitive capacity to follow simple verbal instructions was necessary and was verified with the Mini-Mental State Examination [60].

Exclusion criteria for both groups were: The subject was not ambulating prior to stroke or SCI, or met one or more of the standard exclusion criteria for Lokomat training (body weight greater than 130 kg, body height greater than 2 m, leg length difference greater than 2 cm, osteoporosis, instable fracture in lower extremity, restricted range of motion, presence of decubitus ulcer of lower extremity). Also, any of the following obstructive diseases limiting training led to exclusion of the study: arthritis causing pain while stepping; dyspnea or angina on moderate exertion; limited walking endurance due to cardiopulmonary or other diseases. Prevalence of other neurological or orthopedic injuries and medical diseases which may limit exercise participation or impair locomotion (e.g. serious infection; severe orthostatic hypotension or uncontrolled hypertension, congestive heart failure, pain while weight-bearing) as well as severe metabolic diseases, epilepsy, pre-morbid ongoing major depression or psychosis were additional exclusion criteria. Subjects were not allowed to participate in other training studies or perform physical therapy interventions targeting the lower limbs during the trial.

The iSCI subjects were recruited using the database of the University Hospital Balgrist, Zurich, Switzerland. Stroke subjects were recruited from the "Zentrum für ambulante Therapie ZAR", Zurich, Switzerland.

Six subjects (two SCI and four stroke subjects) were recruited for the trial (Table 1). However, two subjects dropped out of the trial after one and two weeks, respectively, because of personal reasons, so that only four subjects completed the trial.

Table 1 Characteristics of subjects recruited for the pilot trial (AIS - ASIA impairment scale [56], WISCI II - Walking Index for Spinal Cord Injury, version II [61, 62])

Training protocol

The subjects trained for 45 min (actual training time) four times a week during a period of four weeks, i.e. each subject performed 16 training sessions in total.

The first training session focused on the subject’s setup and adjustments within the device and was carried out by two therapists to reduce setup time. To allow subjects to acclimatize, training started with approximately 30% body-weight support and a treadmill speed of 1.9 km/h. All subjects initially started training using foot lifters (stroke subjects only at the affected side) to ensure foot clearance during swing phase. If control and strength of ankle dorsiflexion improved, the tension of the foot lifters was decreased until the point of volitional dorsiflexion was sufficient to remove the foot lifters.

In subsequent sessions, training intensity was increased progressively by changing walking speed, level of body-weight support and guidance force of the robot. The amount of BWS was adjusted individually in order for the subjects to achieve adequate knee extension during the stance phase and toe clearance during the swing phase. Adjustments were made according to the following priorities:

  1. 1.

    The stiffness of the impedance controller (guidance force) was decreased as far as possible, until 0% guidance force was reached and the subject was training only with the Generalized Elastic Path Control strategy.

  2. 2.

    The tunnel width was increased from narrow as far as possible until the widest tunnel setting was reached.

  3. 3.

    Treadmill speed was increased as far as possible until the maximal speed of 4.0 km/h was reached.

  4. 4.

    Body-weight support was reduced as much as possible.

The adjustments listed above were made by the therapist continuously during each session. Based on experience in manual and robot-aided gait rehabilitation, the therapist subjectively judged gait quality and the subject’s level of motivation to decide if the subject was likely to tolerate a more challenging training. The goal for each training session was to reach the highest level of challenge, i.e. a guidance force of 0%, the widest tunnel setting, a treadmill speed of 4.0 km/h and the lowest level of body-weight support tolerated by the patient. Adaptations were always done systematically, following the order stated above.

If subjects were too exhausted to continue, which was judged by the therapist who subjectively assessed the quality of their walking patterns, training intensity was reduced by taking back the adjustments in reverse order. When subjects needed to be distracted from exhaustion towards the end of the 45 min of training, the visual feedback was changed to a coin-collecting game from the commercial "Lokomat System Augmented Feedback" [63].

When subjects were able to walk with the widest tunnel setting, we tested whether they were able to control the treadmill in Automatic Treadmill Speed Adaptation mode. If this was the case, 10 to 15 min of Automatic Treadmill Speed Adaptation were introduced in the training sessions to increase variability of the training and active participation of the subjects.

Outcome measures and data recording

In the second training session (baseline), after two weeks of training and in the final training session after four weeks, we performed electromyography (EMG) of five leg muscles (rectus femoris (RF), vastus medialis (VM), biceps femoris (BF), tibialis anterior (TA) and gastrocnemius medialis (GM)) as well as heart rate measurements under five different training conditions:

  1. 1.

    Walking on the treadmill with body-weight support but without the Lokomat (Free).

  2. 2.

    Walking with the Lokomat in Generalized Elastic Path Control mode (0% guidance force), with the maximal tunnel width (Wide).

  3. 3.

    Walking with the Lokomat in Generalized Elastic Path Control mode (0% guidance force), with the minimal tunnel width (Narrow).

  4. 4.

    Walking with the Lokomat in Generalized Elastic Path Control mode (0% guidance force), with the minimal tunnel width and an additional supportive flow of k sup = 5Nm (Narrow +).

  5. 5.

    Walking with the Lokomat in position control mode, i.e. with the impedance controller set to 100% guidance force (Pos).

The level of body-weight support was set to one third of the subject’s body mass under all conditions, which proved to be sufficient for all subjects. The subjects walked for 2 min under each condition, while signals were recorded. After 90 s, they were questioned about their subjective feeling of effort (perceived exertion) using the Borg-Scale ranging from 6 to 20 [64, 65]. Conditions were applied in randomized order, and patients were not informed about the order of the conditions.

EMG recordings were conducted according to the SENIAM guidelines [66]. In iSCI subjects, EMG signals were recorded from the weaker leg, which was determined after muscle testing according to the ASIA motor score [67]. In stroke subjects, the data was collected from the affected paretic leg. Signals were recorded with 1000 Hz and band-pass filtered between 30 to 300 Hz with an additional notch filter at 50 Hz.

The outcome measure to investigate the effects of training was the ten meter walking test (TMWT) [68], which assesses the time needed by the subject to ambulate 10 m. Both, self-selected velocity (SSV) and fast velocity (FV) of the subjects were determined, with the instruction to "walk at your normal, comfortable pace" and to "walk as fast as safely possible", respectively. The TMWT was performed once before the start of the trial and then again after every week of training, leading to a total of five measurements.

Data analysis

EMG data

In order to classify the quantity and quality of muscle activity with different control strategies for the Lokomat, we computed an EMG metric according to [69]. This metric has been developed and validated for leg muscle activity during walking and assesses the similarity to a pattern of norm activity. To obtain patterns of norm activity for the muscles of interest, we recorded EMG data from 16 healthy subjects walking on a treadmill without the Lokomat (Table 2).

Table 2 Healthy subjects for obtaining EMG reference data

The recorded EMG signals were rectified and cut into single strides triggered by the heel strike signal of the force sensors of the treadmill. The single strides were normalized in time to 1000 samples each. All strides of a subject were then averaged. The EMG amplitudes were normalized to one, by dividing the signals by the maximal signal level recorded for each muscle. Finally, the average activity patterns of the single subjects were averaged to obtain the patterns of norm activity for each muscle.

From the patterns of norm activity, binary on/off patterns were extracted by assigning the value "1" (representing "on") to phases above a defined threshold thresholdmuscle and to "−1" (representing "off") for phases below threshold (Figure 4). The value for thresholdmuscle was set to 15% of the maximum of the averaged muscle activity:

NormAct(S)= 1 EMG signal > threshold muscle 1 EMG signal threshold muscle

Averaged EMG signals of the subjects obtained under the different conditions mentioned above were then related to the norm activity patterns by two heuristics, one for the magnitude of the EMG signal, and one for the phase of the EMG signal [69]. The values obtained by the heuristics are confined to the interval [0,1]. Briefly summarized, the magnitude component yields a maximal value of 1 if the EMG signal has maximal amplitude during the "on" phases and zero amplitude during the "off" phases of the norm pattern. The phase component yields a maximal value of 1 if the EMG signal is always above threshold during the "on" phases and always below threshold during the "off" phases of the norm pattern. Magnitude components and phase components for all muscles are averaged to obtain one value which represents the similarity of overall muscle activity to the pattern of norm activity. Because of the simplified rectangular shape of the norm pattern, the amplitude component tends to yield values lower than 1 for physiological activity patterns. Based on exemplary data [69] and a validation experiment with a subset of the subjects listed in Table 2, we can expect EMG metric values of 0.7–0.8 for healthy subjects walking freely on a treadmill.

Figure 4
figure 4

Patterns of EMG norm activity over one gait cycle ranging from one initial contact (0% gait cycle) to the next initial contact (100% gait cycle). The dashed line represents the average muscle activity recorded from healthy subjects S01–S16 during treadmill walking. The solid line represents the discrete "on"/"off" signal NormAct(S).

The EMG measurements were repeated three times (at baseline, after two weeks, and after four weeks). To obtain one representative value for each subject under each condition, we calculated the median of the EMG metric for these three separate measurements. As we were also interested in potential trends in the EMG metric over the course of the four weeks of training, we normalized the muscle activity of the subjects to the maximal EMG value of the average stride under the free walking condition during their first (baseline) measurement session.

To compare the EMG metrics obtained under the different conditions, we performed a Friedman test at the 5% significance level. In subsequent post-hoc tests, we applied the Bonferroni adjustment for multiple comparisons [70, 71].

Physical effort

We normalized the heart rate values HRduring of the subjects during walking under the different Lokomat conditions to their heart rate HRfree, which we recorded while they were walking on the treadmill without the Lokomat (condition Free):

HR rel = Hr during HR free .

As a measure of perceived physical effort, we asked subjects to rate their level of exertion on the Borg scale, which ranges from 6 to 20 [65, 72], after 90 s of walking under the respective condition.

Analogous to the EMG metric, the median value of the three measurements (at baseline, after two weeks, and after four weeks) was calculated for relative heart rate HRrel and Borg scale. Statistical comparisons of the different conditions were performed with a Friedman test at the 5% significance level and Bonferroni adjustment for multiple comparisons during post-hoc tests [70, 71].


To eliminate the effect of day to day fluctuations in the TMWT, we fit a linear model to the measured walking speeds of each subject:

v TMWT = β 0 + β 1 × n weeks 4 .

In this model, β0corresponds to the initial walking speed of a subject at baseline, while β1 reflects the increase in walking speed after the four weeks of training in the pilot trial. The resulting model coefficients β0 and β1 were checked by t-tests (α = 0.05) for a significant difference to zero.


Comparison of different training modes

The EMG patterns of our subjects showed the highest similarity to the pattern of norm activity under condition Free, i.e. while they were walking on the treadmill without the Lokomat. The value of the EMG metric was significantly lower under condition Pos. There were no significant differences between other conditions (Figure 5).

Figure 5
figure 5

EMG metric of subjects under different conditions.

No significant differences between conditions were identified for relative heart rate (Figure 6) and Borg scale (Figure 7). However, for the Borg scale, a distinct trend towards reduced perceived exertion during condition Pos compared to the other conditions was visible.

Figure 6
figure 6

Heart rate of subjects under different conditions.

Figure 7
figure 7

Borg scale of subjects under different conditions.

The dashed line represents the average muscle activity recorded from healthy subjects S01–S16 during treadmill walking. The solid line represents the discrete "on"/"off" signal NormAct(S).


In all iSCI subjects, the results of the TMWT fluctuated excessively from measurement to measurement (Figure 8).

Figure 8
figure 8

Results of ten meter walking test and regression model fit for each subject and for preferred, self-selected (SSV, left column) and fast (FV, right column) walking velocity.

Subject P04 showed a consistent improvement in walking speed over the four weeks of training, which is also reflected in the coefficients of the linear regression model fit (Table 3). The modeled increase in walking velocity during the training period was 0.16 m/s for the SSV and 0.167 m/s for the FV. The increase in SSV contributed significantly to the linear model (p = 0.024), while the increase in FV was distinct but just not significant (p = 0.065).

Table 3 Linear regression model coefficients of results in ten meter walking test

For subjects P01, P03, and P06, the modeled changes in walking speed were small (| β 1 | 0 . 04 m/s 4 weeks ) and did not contribute significantly to the linear model (p(β1) ≥ 0.4).


Feasibility of patient-cooperative robot-aided gait training

The patient-cooperative robot-aided gait training with the Generalized Elastic Path Control approach was tolerated well by all subjects and was performed without difficulties. One important aspect which contributed substantially to the feasibility was the combination of the Generalized Elastic Path Control approach with the impedance control approach. Especially during the first training sessions, subjects started with full guidance of the impedance controller, and the gradual decrease of guidance force allowed them to slowly get used to training with more active participation.

Despite using a fixed treadmill speed that was not directly influenced by the subjects, the freedom of timing in swing phase made it a constant challenge to keep the timing of walking synchronized with the virtual "ghost legs" of the avatar shown to the subjects. All patients quickly understood this task and mastered it with varying success during the single training sessions, typically with good initial performance which reduced when the level of challenge was increased, and when subjects got more exhausted.

In the subjective observation of the therapist, all subjects became more confident in influencing their gait pattern with the Lokomat over the four weeks of training.

Short-term reaction to patient-cooperative vs. non-cooperative training

Our second research question aimed at identifying the different short-term reactions of patients to different control strategies for the Lokomat, and more specifically, to patient-cooperative vs. non-cooperative control strategies.

Despite the limited statistical power associated with our small number of subjects of n = 4, our results clearly show that the non-cooperative position control mode causes subjects to activate their leg muscles in a way that is significantly less similar to a physiological activation pattern than when they are walking on a treadmill without the Lokomat (Figure 5). These findings are consistent with earlier studies by other groups investigating the influence of (non-cooperative) robot-aided gait training with the Lokomat [73, 74]. In contrast, muscle activity under all patient-cooperative Lokomat conditions was more similar to the norm activation pattern than under the non-cooperative condition.

Interestingly, the effects of the different control modes on muscle activity are well in line with the trend visible in the results of the Borg scale rating of the perceived exertion of the subjects (Figure 7). The human motor system uses every possibility to "slack" [75], and it seems that the additional pressure of the challenging rehabilitation training causes subjects to relieve this pressure by relying strongly on the unconditional support of the non-cooperative position control mode. The patient-cooperative modes (independent of tunnel width or the presence of an additional flow of support) appear to keep subjects sufficiently active to exhibit near-to-normal muscle activity and perceived exertion.

These findings extend the results reported in [51], which only had investigated the effects of patient-cooperative robot-aided training on the quantity of muscle activity, by showing that the patient-cooperative control modes also improve the quality of the patterns of muscle activity. Impedance control [38] with medium controller stiffness did not show significantly increased levels of muscle activity in this previous investigation [51], whereas impedance control with low controller stiffness has not been practical with the Lokomat, as the effects of inertia cause perturbations and result in a non-physiological gait pattern. As a consequence, we focused the present investigations on the patient-cooperative Path Control approach that improves the transparency of the device so that natural walking with more freedom becomes possible.

Thus, the patient-cooperative control mode investigated in the study allows patients to train more actively and—as a consequence—with more physiological muscle activity than the established non-cooperative position control mode. Patients are aware of their increased activity, a fact which could contribute positively to their motivation.

Effect of training on walking function

The last research question addressed the effect of four weeks of patient-cooperative robot-aided gait training on walking function. More specifically, we were interested in changes in walking speed in the TMWT. Only one subject (P04) showed a significant change in this outcome measure, which increased by 0.16 m/s (SSV).

In comparison to results obtained by other studies (Table 4), this improvement distinctly exceeds the gains in walking speed of 0.05–0.07 m/s typically achieved in four weeks of non-cooperative Lokomat training.

Table 4 Improvements in walking speed (SSV) in clinical trials evaluating robot-aided gait training with the Lokomat

Subjects P01, P03, and P06 did not significantly change their overground walking speed, even though the therapists subjectively reported that the subjects visibly improved their gait in the Lokomat. P01 and P06 were older than the other subjects (both 69 years old) and did not seem to be sufficiently flexible in their behavior to modify their accustomed compensatory walking strategies for daily life. Subject P03 showed a remarkable improvement during the first two weeks of training, but fell back during the second two weeks (Figures 8c and 8d).

The limited number of subjects in this pilot trial does not permit valid conclusions on the effect of patient-cooperative robot-aided gait training on walking function. With respect to a potential larger randomized controlled trial, several aspects should be considered: Even though chronic subjects are attractive for pilot trials because all improvement can be attributed to the intervention, it is questionable whether they constitute a meaningful population for assessing the realistic potential of a specific rehabilitation intervention.

As it very likely happened in two of the subjects in this pilot trial, improvements may just not take place if established compensation strategies are already so deeply engrained that an additional behavioral intervention would be necessary in addition to the motor training. Therefore, we need to start understanding why specific patients respond to certain interventions and others don’t in order to tailor robot-aided therapy much more to their needs. Modeling recovery based on data from larger trials as e.g. done with data from the EXCITE trial for upper extremity stroke rehabilitation [77] may be an important first step in this direction.

Moreover, to obtain a better understanding of the specific effects of the training of single subjects in detail, and be ultimately able to link the success or failure of specific interventions to the precise nature of specific deficits, data acquisition during the trials should be extended to include more parameters such as step lengths, detailed ground reaction forces, and ideally even trunk movements.

Thus, larger clinical trials investigating the effects of robot-aided therapy interventions should focus rather on acute and subacute patient populations and tailor the interventions to the specific needs and deficits that an individual patient needs to overcome in order to reach the threshold of function required for increasing the use of the affected limb in everyday life [78].


Patient-cooperative, robot-aided gait training with stroke and iSCI patients is feasible—not only for single training sessions, as demonstrated previously [51], but also over the course of a realistic training period of four weeks. Patients do not only participate more actively than during non-cooperative, position-controlled robot-aided gait training, they also show more physiological patterns of muscle activity in their main leg muscles.

Thus, patient-cooperative robot-aided gait training overcomes the main points of criticism against robot-aided gait training: It enables patients to train walking in an active, variable and more natural way. However, the question remains open how much more effective robot-aided gait training may become due to these improvements. One of the chronic subjects in this trial showed very encouraging results, whereas the others were not receptive to the therapy. In this respect, an important topic of future rehabilitation research should be the question what makes certain patients respond to a specific rehabilitation intervention while others don’t.

A large, possibly multi-center randomized controlled clinical trial is required to shed more light on this question. In contrast to previous trials, robot-aided training tasks should be systematically tailored to the specific needs of the individual patients, based on e.g. computational models of patient recovery driven by data from clinical and robot-aided assessment. Distributing the proposed patient-cooperative control algorithms to a substantial fraction of the more than 300 Lokomat robots in clinical use world-wide may provide an important basis for such a trial.




  1. Lennon S: The Bobath concept: a critical review of the theoretical assumptions that guide physiotherapy practice in stroke rehabilitation. Phys Therapy Rev. 1996, 35-45.

    Google Scholar 

  2. Seligman MEP: Helplessness: On Depression, Development, and Death. 1975, San Francisco: WH Freeman

    Google Scholar 

  3. Taub E, Miller NE, Novack TA, Cook EW, Fleming WC, Nepomuceno CS, Connell JS, Crago JE: Technique to improve chronic motor deficit after stroke. Arch Phys Med Rehabilitation. 1993, 74 (4): 347-354.

    CAS  Google Scholar 

  4. Wolf SL, Winstein CJ, Miller JP, Taub E, Uswatte G, Morris D, Giuliani C, Light KE, Nichols-Larsen D, for the EXCITE Investigators: Effect of constraint-induced movement therapy on upper extremity function 3 to 9 months after stroke: the EXCITE randomized clinical trial. JAMA. 2006, 296 (17): 2095-2104. 10.1001/jama.296.17.2095.

    Article  CAS  PubMed  Google Scholar 

  5. Wolf SL, Winstein CJ, Miller JP, Thompson PA, Taub E, Uswatte G, Morris D, Blanton S, Nichols-Larsen D, Clark PC: Retention of upper limb function in stroke survivors who have received constraint-induced movement therapy: the EXCITE randomised trial. Lancet Neurol. 2008, 7 (1): 33-40. 10.1016/S1474-4422(07)70294-6.

    Article  PubMed Central  PubMed  Google Scholar 

  6. Lin K-C, Wu C-Y, Liu J-S, Chen Y-T, Hsu C-J: Constraint-induced therapy versus dose-matched control intervention to improve motor ability, basic/extended daily functions, and quality of life in stroke. Neurorehabil Neural Repair. 2009, 23 (2): 160-165.

    Article  PubMed  Google Scholar 

  7. Maier IC, Baumann K, Thallmair M, Weinmann O, Scholl J, Schwab ME: Constraint-induced movement therapy in the adult rat after unilateral corticospinal tract injury. J Neurosci. 2008, 28 (38): 9386-9403. 10.1523/JNEUROSCI.1697-08.2008.

    Article  CAS  PubMed  Google Scholar 

  8. Barbeau H, Wainberg M, Finch L: Description and application of a system for locomotor rehabilitation. Med Biol Eng Comput. 1987, 25 (3): 341-344. 10.1007/BF02447435.

    Article  CAS  PubMed  Google Scholar 

  9. Barbeau H, Visintin M: Optimal outcomes obtained with body-Weight support combined with treadmill training in stroke subjects. Arch Phys Med Rehabilitation. 2003, 84 (10): 1458-1465. 10.1016/S0003-9993(03)00361-7.

    Article  Google Scholar 

  10. Barbeau H, Nadeau S, Garneau C: Physical determinants, emerging concepts, and training approaches in gait of individuals with spinal cord injury. J Neurotrauma. 2006, 23 (3-4): 571-585. 10.1089/neu.2006.23.571.

    Article  PubMed  Google Scholar 

  11. Cai LL, Fong AJ, Otoshi CK, Liang Y, Burdick JW, Roy RR, Edgerton VR: Implications of assist-as-needed robotic step training after a complete spinal cord injury on intrinsic strategies of motor learning. J Neurosci. 2006, 26 (41): 10564-10568. 10.1523/JNEUROSCI.2266-06.2006.

    Article  CAS  PubMed  Google Scholar 

  12. Dromerick A, Lum P, Hidler J: Activity-based therapies. NeuroRX. 2006, 3 (4): 428-438. 10.1016/j.nurx.2006.07.004.

    Article  PubMed Central  PubMed  Google Scholar 

  13. Norman K, Pepin A, Ladouceur M, Barbeau H: A treadmill apparatus and harness support for evaluation and rehabilitation of gait. Arch Phys Med Rehabilitation. 1995, 76 (8): 772-778. 10.1016/S0003-9993(95)80533-8.

    Article  CAS  Google Scholar 

  14. Visintin M, Barbeau H, Korner-Bitensky N, Mayo NE: A new approach to retrain gait in stroke patients through body weight support and treadmill stimulation. Stroke; J Cerebral Circulation. 1998, 29 (6): 1122-1128. 10.1161/01.STR.29.6.1122.

    Article  CAS  Google Scholar 

  15. Dobkin B, Apple D, Barbeau H, Basso M, Behrman A, Deforge D, Ditunno J, Dudley G, Elashoff R, Fugate L, Harkema S, Saulino M, Scott M: Weight-supported treadmill vs over-ground training for walking after acute incomplete SCI. Neurology. 2006, 66 (4): 484-493. 10.1212/01.wnl.0000202600.72018.39.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  16. van Hedel Hubertus JA: Weight-supported treadmill versus over-ground training after spinal cord injury: from a physical therapist’s point of view. PHYS THER. 2006, 86 (10): 1444-1447. 10.2522/ptj.2006.86.10.1444.

    Article  CAS  PubMed  Google Scholar 

  17. Sullivan KJ, Brown DA, Klassen T, Mulroy S, Ge T, Azen SP, Winstein CJ: Effects of task-specific locomotor and strength training in adults who were ambulatory after stroke: results of the STEPS randomized clinical trial. Phys Therapy. 2007, 87 (12): 1580-1602. 10.2522/ptj.20060310.

    Article  Google Scholar 

  18. Colombo G, Joerg M, Schreier R, Dietz V: Treadmill training of paraplegic patients using a robotic orthosis. J Rehabil Res Dev. 2000, 37 (6): 693-700.

    CAS  PubMed  Google Scholar 

  19. Riener R, Lünenburger L, Maier I, Colombo G, Dietz V: Locomotor training in subjects with sensori-motor deficits: an overview of the robotic gait orthosis Lokomat. J Healthcare Eng. 2010, 1 (2): l216-

    Article  Google Scholar 

  20. Hesse S, Uhlenbrock D: A mechanized gait trainer for restoration of gait. J Rehabilitation Res Dev. 2000, 37 (6): 701-708.

    CAS  Google Scholar 

  21. Sayers SP, Krug J: Robotic gait-assisted therapy in patients with neurological injury. Missouri Med. 2008, 105 (2): 153-158.

    PubMed  Google Scholar 

  22. Schmidt H, Werner C, Bernhardt R, Hesse S, Krüger Jörg: Gait rehabilitation machines based on programmable footplates. J Neuroengineering Rehabilitation. 2007, 4: 2+-10.1186/1743-0003-4-2.

    Article  Google Scholar 

  23. Veneman JF, Kruidhof R, Hekman EEG, Ekkelenkamp R, Van Asseldonk EHF, van der Kooij H: Design and evaluation of the LOPES exoskeleton robot for interactive gait rehabilitation. IEEE Trans Neural Syst Rehabil Eng. 2007, 15 (3): 379-386.

    Article  PubMed  Google Scholar 

  24. Banala SK, Agrawal SK, Scholz JP: Active Leg Exoskeleton (ALEX) for gait rehabilitation of motor-impaired patients. Proc IEEE 10th Int Conf Rehabil Robot. 2007, Noordwijk, 401-407.

    Google Scholar 

  25. Aoyagi D, Ichinose WE, Harkema SJ, Reinkensmeyer DJ, Bobrow JE: A robot and control algorithm that can synchronously assist in naturalistic motion during body-weight-supported gait training following neurologic injury. IEEE Trans Neural Syst Rehabil Eng. 2007, 15 (3): 387-400.

    Article  PubMed  Google Scholar 

  26. Dollar AM, Herr H: Lower extremity exoskeletons and active orthoses: challenges and state-of-the-art. Robotics IEEE Trans on. 2008, 24 (1): 144-158.

    Article  Google Scholar 

  27. Mehrholz J, Werner C, Kugler J, Pohl M: Electromechanical-assisted training for walking after stroke. Cochrane Database of Systematic Rev (Online). 2007, 4

    Google Scholar 

  28. Mehrholz J, Kugler J, Pohl M: Locomotor training for walking after spinal cord injury. Cochrane Database of Systematic Rev (Online). 2008, 2

    Google Scholar 

  29. Husemann B, Müller F, Krewer C, Heller S, Koenig E: Effects of locomotion training with assistance of a robot-driven gait orthosis in hemiparetic patients after stroke: a randomized controlled pilot study. Stroke; J Cerebral Circulation. 2007, 38 (2): 349-354. 10.1161/01.STR.0000254607.48765.cb.

    Article  Google Scholar 

  30. Mayr A, Kofler M, Quirbach E, Matzak H, Frohlich K, Saltuari L: Prospective, blinded, randomized crossover study of gait rehabilitation in stroke patients using the lokomat gait orthosis. Neurorehabil Neural Repair. 2007, 21 (4): 307-314. 10.1177/1545968307300697.

    Article  PubMed  Google Scholar 

  31. Schwartz I, Sajin A, Fisher I, Neeb M, Shochina M, Katz-Leurer M, Meiner Z: The effectiveness of locomotor therapy using robotic-assisted gait training in subacute stroke patients: a randomized controlled trial. PM R : J Injury, Funct, Rehabilitation. 2009, 1 (6): 516-523. 10.1016/j.pmrj.2009.03.009.

    Article  Google Scholar 

  32. Westlake Kelly, Patten C: Pilot study of Lokomat versus manual-assisted treadmill training for locomotor recovery post-stroke. J NeuroEngineering Rehabilitation. 2009, 6 (1): 18+-10.1186/1743-0003-6-18.

    Article  Google Scholar 

  33. Hidler J, Nichols D, Pelliccio M, Brady K, Campbell DD, Kahn JH, Hornby GT: Multicenter randomized clinical trial evaluating the effectiveness of the Lokomat in subacute stroke. Neurorehabil Neural Repair. 2009, 23 (1): 5-13.

    Article  PubMed  Google Scholar 

  34. Hornby GT, Campbell DD, Kahn JH, Demott T, Moore JL, Roth HR: Enhanced gait-related improvements after therapist- versus robotic-assisted locomotor training in subjects with chronic stroke: a randomized controlled study. Stroke. 2008, 39 (6): 1786-1792. 10.1161/STROKEAHA.107.504779.

    Article  PubMed  Google Scholar 

  35. Murray AF, Edwards PJ: Synaptic weight noise during MLP learning enhances fault-tolerance, generalisation and learning trajectory. IEEE Trans on Neural Networks. 1993, 5 (5): 792-802.

    Article  Google Scholar 

  36. Bernstein NA: The Co-ordination and regulation of movements. 1967, First English edition. Pergamon Press Ltd.

    Google Scholar 

  37. Hogan N: Impedance control - an approach to manipulation. I - Theory. II - Implementation. III - Applications. ASME Trans J Dynamic Syst Meas Control B. 1985, 107: 1-24. 10.1115/1.3140702.

    Article  Google Scholar 

  38. Riener R, Lünenburger L, Jezernik S, Anderschitz M, Colombo G, Dietz V: Patient-cooperative strategies for robot-aided treadmill training: first experimental results. IEEE Trans Neural Syst Rehabil Eng. 2005, 13 (3): 380-394. 10.1109/TNSRE.2005.848628.

    Article  PubMed  Google Scholar 

  39. Emken JL, Bobrow JE, Reinkensmeyer DJ: Robotic movement training as an optimization problem: designing a controller that assists only as needed. IEEE Int. Conf. on Rehabilitation Robotics (ICORR). 2005, Chicago, 307-312.

    Google Scholar 

  40. Reinkensmeyer DJ, Emken JL, Cramer SC: Robotics, motor learning, and neurologic recovery. Ann Rev Biomed Eng. 2004, 6: 497-525. 10.1146/annurev.bioeng.6.040803.140223.

    Article  CAS  Google Scholar 

  41. Riener R, Fuhr T: Patient-driven control of FES-supported standing up: a simulation study. Rehabilitation Eng, IEEE Trans on. 1998, 6 (2): 113-124. 10.1109/86.681177.

    Article  CAS  Google Scholar 

  42. Jezernik S, Colombo G, Morari M: Automatic gait-pattern adaptation algorithms for rehabilitation with a 4-DOF robotic orthosis. IEEE Trans Robot Autom. 2004, 20 (3): 574-582. 10.1109/TRA.2004.825515.

    Article  Google Scholar 

  43. Riener R, Frey M, Bernhardt M, Nef T, Colombo G: Human-centered rehabilitation robotics. Rehabilitation Robotics, 2005. ICORR 2005. 9th International Conference on. 2005, 319-322.

    Chapter  Google Scholar 

  44. Reinkensmeyer DJ, Aoyagi D, Emken JL, Galvez JA, Ichinose W, Kerdanyan G, Maneekobkunwong S, Minakata K, Nessler JA, Weber R, Roy RR, de Leon R, Bobrow JE, Harkema SJ, Edgerton VR: Tools for understanding and optimizing robotic gait training. J Rehabil Res Dev. 2006, 43 (5): 657-670. 10.1682/JRRD.2005.04.0073.

    Article  PubMed  Google Scholar 

  45. van Asseldonk EHF, Veneman JF, Ekkelenkamp R, Buurke JH, van der Helm FCT, van der Kooij H: The effects on kinematics and muscle activity of walking in a robotic gait trainer during zero-force control. IEEE Trans Neural Syst Rehabil Eng. 2008, 16 (4): 360-370.

    Article  PubMed  Google Scholar 

  46. Vallery H, Guidali M, Duschau-Wicke A, Riener R: Patient-cooperative control: providing safe support without restricting movement. World Congress on Medical Physics and Biomedical Engineering, September 7 - 12, 2009, Munich, Germany. Edited by: Dössel Olaf, Schlegel WolfgangC. 2009, Berlin, Heidelberg: Springer Berlin Heidelberg, 166-169.

    Chapter  Google Scholar 

  47. Emken JL, Harkema SJ, Beres-Jones JA, Ferreira CK, Reinkensmeyer DJ: Feasibility of manual teach-and-replay and continuous impedance shaping for robotic locomotor training following spinal cord injury. IEEE Trans Biomed Eng. 2008, 55 (1): 322-334.

    Article  PubMed  Google Scholar 

  48. Marchal Crespo L, Reinkensmeyer D: Review of control strategies for robotic movement training after neurologic injury. J Neuroengineering rehabilitation. 2009, 6 (1): 20+-10.1186/1743-0003-6-20.

    Article  Google Scholar 

  49. Cai LL, Fong AJ, Otoshi CK, Liang YQ, Cham JG, Zhong H, Roy RR, Edgerton VR, Burdick JW: Effects of consistency vs. variability in robotically controlled training of stepping in adult spinal mice. 2005, Chicago

    Chapter  Google Scholar 

  50. Duschau-Wicke A, von Zitzewitz J, Caprez A, Lünenburger L, Riener R: Path control: a method for patient-cooperative robot-aided gait rehabilitation. IEEE Trans Neural Syst Rehabilitation Eng. 2010, 18 (1): 38-48.

    Article  Google Scholar 

  51. Duschau-Wicke A, Caprez A, Riener R: Patient-cooperative control increases active participation of individuals with SCI during robot-aided gait training. J NeuroEngineering Rehabilitation. 2010, 7 (1): 43+-10.1186/1743-0003-7-43.

    Article  Google Scholar 

  52. Frey M, Colombo G, Vaglio M, Bucher R, Jörg M, Riener R: A novel mechatronic body weight support system. IEEE Trans Neural Syst Rehabil Eng. 2006, 14 (3): 311-321. 10.1109/TNSRE.2006.881556.

    Article  PubMed  Google Scholar 

  53. Vallery H, Duschau-Wicke A, Riener R: Generalized elasticities improve patient-cooperative control of rehabilitation robots. IEEE Int Conf on Rehabilitation Robotics (ICORR). 2009, 535-541.

    Google Scholar 

  54. Vallery H, Duschau-Wicke A, Riener R: Optimized passive dynamics improve transparency of haptic devices. IEEE Int Conf Robot Aut (ICRA). 2009, 301-306.

    Google Scholar 

  55. von Zitzewitz J, Bernhardt M, Riener R: A Novel method for automatic treadmill speed adaptation. IEEE Trans Neural Syst Rehabil Eng. 2007, 15 (3): 401-409.

    Article  PubMed  Google Scholar 

  56. Maynard FM, Bracken MB, Creasey G, Ditunno JF, Donovan WH, Ducker TB, Garber SL, Marino RJ, Stover SL, Tator CH, Others: International standards for neurological and functional classification of spinal cord injury. Spinal Cord. 1997, 35: 266-274. 10.1038/

    Article  PubMed  Google Scholar 

  57. Itzkovich M, Gelernter I, Biering-Sorensen F, Weeks C, Laramee MT, Craven BC, Tonack M, Hitzig SL, Glaser E, Zeilig G, Aito S, Scivoletto G, Mecci M, Chadwick RJ, Masry WS, Osman A, Glass CA, Silva P, Soni BM, Gardner BP, Savic G, Bergström EM, Bluvshtein V, Ronen J, Catz A: The Spinal Cord Independence Measure (SCIM) version III: reliability and validity in a multi-center international study. Disabil Rehabil. 2007, 29 (24): 1926-1933. 10.1080/09638280601046302.

    Article  CAS  PubMed  Google Scholar 

  58. Cuthbert SC, Goodheart GJ: On the reliability and validity of manual muscle testing: a literature review. Chiropractic Osteopathy. 2007, 15: 4+-10.1186/1746-1340-15-4.

    Article  PubMed Central  PubMed  Google Scholar 

  59. Schmitt WH, Cuthbert SC: Common errors and clinical guidelines for manual muscle testing: "the arm test" and other inaccurate procedures. Chiropractic Osteopathy. 2008, 16: 16+-10.1186/1746-1340-16-16.

    Article  PubMed Central  PubMed  Google Scholar 

  60. Cockrell JR, Folstein MF: Mini-mental state examination. Principles Pract Geriatric Psychiatry. 1988, 140-141.

    Google Scholar 

  61. Dittuno PL, Dittuno JF: Walking index for spinal cord injury (WISCI II): scale revision. Spinal Cord. 2001, 39: 654-656. 10.1038/

    Article  CAS  PubMed  Google Scholar 

  62. van Hedel HJ, Dietz V, European multicenter study on human spinal cord injury EM-SCI study group: Walking during daily life can be validly and responsively assessed in subjects with a spinal cord injury. Neurorehabilitation Neural Repair. 2009, 23 (2): 117-124.

    Article  PubMed  Google Scholar 

  63. Zimmerli L, Duschau-Wicke A, Mayr A, Riener R, Lünenburger L: Virtual reality and gait rehabilitation Augmented feedback for the Lokomat. Virtual Rehabilitation International Conference. 2009, 150-153.

    Chapter  Google Scholar 

  64. Borg G: Perceived exertion as an indicator of somatic stress. Scand J Rehabilitation Med. 1970, 2 (2): 92-98.

    CAS  Google Scholar 

  65. Lewis JE, Nash MS, Hamm LF, Martins SC, Groah SL: The relationship between perceived exertion and physiologic indicators of stress during graded arm exercise in persons with spinal cord injuries. Arch Phys Med Rehabilitation. 2007, 88 (9): 1205-1211. 10.1016/j.apmr.2007.05.016.

    Article  Google Scholar 

  66. Hermens HJ, Freriks B, Merletti R, Stegeman D, Blok J, Rau G, Disselhorst-Klug C, Haegg G: European Recommendations for Surface Electromyography. 1999

    Google Scholar 

  67. Marino RJ, Barros T, Biering-Sorensen F, Burns SP, Donovan WH, Graves DE, Haak M, Hudson LM, Priebe MM, ASIA Neurological Standards Committee 2002: International standards for neurological classification of spinal cord injury. J Spinal Cord Med. 2003, 26 (Suppl 1):

  68. Alexander MS, Anderson KD, Biering-Sorensen F, Blight AR, Brannon R, Bryce TN, Creasey G, Catz A, Curt A, Donovan W, Ditunno J, Ellaway P, Finnerup NB, Graves DE, Haynes BA, Heinemann AW, Jackson AB, Johnston MV, Kalpakjian CZ, Kleitman N, Krassioukov A, Krogh K, Lammertse D, Magasi S, Mulcahey MJ, Schurch B, Sherwood A, Steeves JD, Stiens S, Tulsky DS, van Hedel HJA, Whiteneck G: Outcome measures in spinal cord injury: recent assessments and recommendations for future directions. Spinal Cord. 2009, 47 (8): 582-591. 10.1038/sc.2009.18.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  69. Ricamato AL, Hidler JM: Quantification of the dynamic properties of EMG patterns during gait. J Electromyography Kinesiology. 2005, 15 (4): 384-392. 10.1016/j.jelekin.2004.10.003.

    Article  Google Scholar 

  70. Gibbons JD: Nonparametric Statistical Inference. 1985, Marcel Dekker Ltd

    Google Scholar 

  71. Hochberg Yosef, Tamhane AjitC: Multiple Comparison Procedures (Wiley Series in Probability and Statistics). 1987, Wiley

    Book  Google Scholar 

  72. Goosey-Tolfrey V, Lenton J, Goddard J, Oldfield V, Tolfrey K, Eston R: Regulating intensity using perceived exertion in spinal cord-injured participants. Med Sci Sports Exercise. 2010, 42 (3): 608-613. 10.1249/MSS.0b013e3181b72cbc.

    Article  Google Scholar 

  73. Hidler JM, Wall AE: Alterations in muscle activation patterns during robotic-assisted walking. Clin Biomech. 2005, 20 (2): 184-193. 10.1016/j.clinbiomech.2004.09.016.

    Article  Google Scholar 

  74. Israel JF, Campbell DD, Kahn JH, Hornby GT: Metabolic costs and muscle activity patterns during robotic- and therapist-assisted treadmill walking in individuals with incomplete spinal cord injury. Phys Therapy. 2006, 86 (11): 1466-1478. 10.2522/ptj.20050266.

    Article  Google Scholar 

  75. Reinkensmeyer DJ, Maier MA, Guigon E, Chan V, Akoner O, Wolbrecht ET, Cramer SC, Bobrow JE: Do robotic and non-robotic arm movement training drive motor recovery after stroke by a common neural mechanism? Experimental evidence and a computational model. Conf. IEEE Engineering in Medicine and Biology Society (EMBS). 2009, 2439-2441.

    Google Scholar 

  76. Wirz M, Zemon DH, Rupp R, Scheel A, Colombo G, Dietz V, Hornby TG: Effectiveness of automated locomotor training in patients with chronic incomplete spinal cord injury: a multicenter trial. Arch Phys Med Rehabil. 2005, 86 (4): 672-680. 10.1016/j.apmr.2004.08.004.

    Article  PubMed  Google Scholar 

  77. Schweighofer N, Han CE, Wolf SL, Arbib MA, Winstein CJ: A functional threshold for long-term use of hand and arm function can be determined: predictions from a computational model and supporting data from the Extremity Constraint-Induced Therapy Evaluation (EXCITE) Trial. Phys therapy. 2009, 89 (12): 1327-1336. 10.2522/ptj.20080402.

    Article  Google Scholar 

  78. Han CE, Arbib MA, Schweighofer N: Stroke rehabilitation reaches a threshold. PLoS Computat Biol. 2008, 4 (8):

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The authors would like to thank all subjects who participated in this study. Furthermore, we are grateful to Markus Wirz, Marc Bolliger, and Huub van Hedel for their support. This work was supported in part by NIDRR grant H133E070013. R. Labruyere was paid by a grant to H. van Hedel from the International Spinal Research Trust (Clinical Initiative Stage II, CLI06).

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Correspondence to Alexander Duschau-Wicke.

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Competing interests

AD is employed by Hocoma AG, Volketswil, Switzerland. Hocoma is the manufacturer of the Lokomat gait rehabilitation robot, which was used as the platform to implement the patient-cooperative control algorithm for robot-aided gait training investigated in this study. AS, RL, HV, and RR declare that they have no competing interests.

Authors’ contributions

AS performed the training and measurements of all subjects, study design and coordination, data analysis, statistical analysis, and drafted the manuscript. RL contributed to the recruitment of subjects and assisted in the measurements and training sessions. HV contributed to the design and implementation of the patient-cooperative control algorithm. RR participated in the design and coordination of the study and assisted with drafting the manuscript. AD contributed to the design and implementation of the patient-cooperative control algorithm, the design and coordination of the study, and assisted with data analysis, statistical analysis and with drafting the manuscript. All authors read and approved the final manuscript.

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Schück, A., Labruyère, R., Vallery, H. et al. Feasibility and effects of patient-cooperative robot-aided gait training applied in a 4-week pilot trial. J NeuroEngineering Rehabil 9, 31 (2012).

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